2012, Number 4
Rev Hosp Jua Mex 2012; 79 (4)
Bravata-Alcántara JC, Ocadiz-Delgado R, Sierra-Martínez M, Reyes-Hernández OD, García-López ES, Acosta-Altamirano G, Manrique MA, Bonilla-Delgado J, Cortés-Malagón EM
PDF size: 238.36 Kb.
ABSTRACTIntroduction. The bacterial pathogen Helicobacter pylori chronically infects the human gastric mucosa and is the leading risk factor for the development of gastric cancer. Material and methods. Helicobacter pylori culture is an invasive, time consuming method, offering quite low sensitivity, requiring significant cost, and which, in practice, tests very few antibiotics, with a questionable contribution to the management of patients. Results. Therefore, we evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes. This multiplex PCR assay would be of particularly great value for molecular diagnosis laboratory of Hospital Juárez de México. Conclusion. In this study the possibility of faster and easier to detect genotypes of Helicobacter pylori associated with gastric cancer, with less time and cost.