2012, Number 4
PDF size: 238.36 Kb.
ABSTRACTIntroduction. The bacterial pathogen Helicobacter pylori chronically infects the human gastric mucosa and is the leading risk factor for the development of gastric cancer. Material and methods. Helicobacter pylori culture is an invasive, time consuming method, offering quite low sensitivity, requiring significant cost, and which, in practice, tests very few antibiotics, with a questionable contribution to the management of patients. Results. Therefore, we evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes. This multiplex PCR assay would be of particularly great value for molecular diagnosis laboratory of Hospital Juárez de México. Conclusion. In this study the possibility of faster and easier to detect genotypes of Helicobacter pylori associated with gastric cancer, with less time and cost.
Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin Microbiol Rev 1997; 10(4): 720-41.
Blaser MJ. Epidemiology and pathophysiology of Campylobacter pylori infections. Rev Infect Dis 1990; 12(Suppl. 1): S99-106.
Leodolter A, et al. A meta-analysis comparing eradication, healing and relapse rates in patients with Helicobacter pyloriassociated gastric or duodenal ulcer. Aliment Pharmacol Ther 2001; 15(12): 1949-58.
Nagini S. Carcinoma of the stomach: A review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol 2012; 4(7): 156-69.
Wen S, Moss SF. Helicobacter pylori virulence factors in gastric carcinogenesis. Cancer Lett 2009; 282(1): 1-8.
Yamazaki S, et al. Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. J Clin Microbiol 2005; 43(8): 3906-16.
Yang JC, et al. Vacuolating toxin gene polymorphism among Helicobacter pylori clinical isolates and its association with m1, m2, or chimeric vacA middle types. Scand J Gastroenterol 1998; 33(11): 1152-7.
Rudi J, et al. Diversity of Helicobacter pylori vacA and cagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases. J Clin Microbiol 1998; 36(4): 944-8.
Rhead JL, et al. A new Helicobacter pylori vacuolating cytotoxin determinant, the intermediate region, is associated with gastric cancer. Gastroenterology 2007; 133(3): 926-36.
Akopyants NS, et al. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol Microbiol 1998; 28(1): 37-53.
van Doorn LJ, et al. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology 1998; 115(1): 58-66.
Yamaoka Y, et al. Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol 1999; 37(7): 2274-9.
Chattopadhyay S, et al. Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens. J Clin Microbiol 2004; 42(6): 2821-4.
Polk DB, Peek RMJr. Helicobacter pylori: gastric cancer and beyond. Nat Rev Cancer 2010; 10(6): 403-14.
Atherton JC, et al. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J Biol Chem 1995; 270(30): 17771-7.
Atherton JC, et al. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 1997; 112(1): 92-9.
Atherton JC. The clinical relevance of strain types of Helicobacter pylori. Gut 1997; 40(6): 701-3.
Lupetti P, et al. Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin. J Cell Biol 1996; 133(4): 801-7.
Morales-Espinosa R, et al. Colonization of Mexican patients by multiple Helicobacter pylori strains with different vacA and cagA genotypes. J Clin Microbiol 1999; 37(9): 3001-4.