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2011, Number 97

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Rev Enfer Infec Pediatr 2011; 24.25 (97)

Identification of Escherichia coli pathogenic genotypes by realtime PCR analysis of antibiotic resistance in the state of Michoacan, Mexico

Ávila CFJ, Jácome GIE, Silva GAR, Silva GO

Language: Spanish
References: 12
Page: 13-16
PDF size: 186.61 Kb.


ABSTRACT

Background: diarrhea syndrome has a very diverse origin, but Escherichia coli is the leading cause of bacterial diarrhea and a reservoir of antimicrobial resistance genes.
Objective: To identify genotypes pathogenic E. coli genes that encode pathogenicity factors. We used the technique of real-time PCR and evaluated the susceptibility of isolated pathogens to antibiotics.
Material and methods: longitudinal, experimental and ambispective involving 100 isolates of clinical samples from the Hospital Infantil de Morelia Eva Samano de Lopez Mateos and the State Public Health Laboratory of Michoacan, Mexico, obtained between September 2009 and March 2010. We used the technique of real-time PCR and evaluated the susceptibility of isolated pathogens to ant biotics
Results: Of the typeable samples, 44% (44 strains) contained at least one gene of pathogenicity, and most were from E. enterotoxigenic coli (50%, 22 strains), followed by E. coli enteroaggregative (41%, 18 strains) and to a lesser extent E. coli enteropathogenic (9%, 4 strains).
Conclusions: The results show a higher incidence of enterotoxigenic Escherichia coli, as has been reported in most of the authors. The less prevalent pathotype was identified with E. coli. The rest of the pathogens was identified genotypes.


REFERENCES

  1. WHO 2004. Global Burden of disease.

  2. Sistema Nacional de Información en Salud.

  3. Manual de enfermedades diarréicas agudas, prevención, control y tratamiento. Secretaria de Salud, 2009.

  4. Guadalupe A. Principales características y diagnóstico de los grupos patógenos de Escherichia coli. Salud Pública de México 2002;44(5):464-475.

  5. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998;11(1):142-201.

  6. Espinoza L. Herramientas molecular. Manual práctico de PCR.17:517-537.

  7. Rodríguez S. Barrera S. La reacción en cadena de la polimerasa a dos décadas de su invención. Ciencia UANL 2004;7(3)

  8. Toma C, Lu Y, Higa N, Nakasone N, Chinen I, Baschkier A, Rivas M, Iwanaga M. Multiplex PCR Assay for identification of human diarrheagenic Escherichia coli. American society of Microbiology 2003;(6)41:2669-2671.

  9. Cortés Ortiz IA, Rodríguez Angeles G, Moreno Escobar EA, Tenorio Lara JM, Torres Mazadiego BP y col. Brote causado por Escherichia coli en Chalco, México. Salud Publica Mex 2002;44:297-302.

  10. Finlay, B. B., I. Rosenshine, M. S. Donnenberg, and J. B. Kaper. 1992. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect. Immun. 60:2541-2543.

  11. Gutiérrez-Jiménez J, Vidal JE, Mejía-Albarrán ME, Resçendiz- Sánchez J, Pérez-Miravete A y col. Prevalencia de patotipos de Escherichia coli diarreogénica en niños del Hospital Infantil de Mexico. Memorias del 34º Congreso Nacional de Microbiología, Asociación Mexicana de Microbiología. 2004.

  12. Anahí Dreser, Veronika J Wirtz, Kitty K Corbett, Gabriela Echániz. Uso de antibióticos en México: revisión de problemas y políticas. Salud pública Méx 2008;50(supl. 4)




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