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2013, Number 1

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Rev Cubana Hig Epidemiol 2013; 51 (1)

Recovery of Shigella spp. preserved on glass beads by dehydration technique

Weng AZ, Echevarría AZ, Maldonado CG, Álvarez MI, Rodríguez MM

Language: Spanish
References: 20
Page: 40-51
PDF size: 248.19 Kb.


ABSTRACT

Introduction: glass bead dehydration technique has been reported as a microbial preservation method for some years, but the available information is limited. Based on this antecedent, it was decided to evaluate its usefulness to preserve bacteria of clinical origin in the collection of microbial cultures at the National Institute of Hygiene, Epidemiology and Microbiology.
Methods: a study was conducted of two Shigella spp. cultures kept under refrigeration. The viability datas obtained during four years of preservation were processed with the statistical package SPSS, version 15.0. Statistical processing included analysis of variance for comparison of the mean recovery values of viable cells for the variables preservation time, dilution and preserving mix, and Scheffe's test of post hoc multiple comparisons for means discrimination. Physiological characteristics were controlled, as well as the response of strains to Gram stain.
Results: no significant differences were found between the preserving mixes used in the trial, and both strains could be recovered during the four years of the study. The protective effect of all mixes was found to decrease after six months. Variability was obtained for some physiological characters (indol, dulcitol and mannitol), but response to Gram stain remained stable in both strains.
Conclusions: the method was found to be suitable to preserve Shigella spp. for a limited time, but it could be used as a short-term preservation alternative in laboratories with few resources, due to its simplicity, low cost and effectiveness. Availability should be ensured of replicas obtained by other techniques.


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