Pantaleón BOS, Triana MME, García MMT

Language: Spanish
References: 14
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ABSTRACT

Introduction: quality control is indispensable in clinical laboratories to offer reliable results.
Objective: to establish the bases to standardize quality control in the haemostasis laboratory.
Methods: a normal plasma mixture obtained from the Provincial Blood Bank of Havana was evaluated. PH, prothrombin time, and activated plasma thromboplastin time were measured to plasma (liquid, frozen and defrosted). It was stored at -20º C, defrosted, strained and lyophilized. To this last one, the pH and the same variables were measured every four months for a year. At a second stage, re-taking and reproducibility was made to the prepared and lyophilized normal plasma and prothrombin time, activated plasma thromboplastin time, fibrinogen dosage, prothrombin time and dosage of VII factor were determined which were also determined in a commercial normal plasma. The average values of each indicator were compared in both plasmas. An evaluation of the plasma was conducted by three external laboratories and local reference values were determined for each trial.
Results: plasma maintained the adequate coagulant activity and pH values for a year. An external evaluation corroborated that the findings in the values were within the reference intervals of the plasma under study. Local reference values were similar to the ones informed by the manufacturers of the reagents.
Conclusions: the evaluated plasma has stability and repeatability so it can be used in quality control. The local reference values for each indicator guarantee the reliability of the results.

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