Cedillo SLC, Banda RVM, Morales SE, Villagómez AE

Language: Spanish
References: 12
Page: 10-21
PDF size: 292.71 Kb.

ABSTRACT

One of the process that directly affects the fertility of milk cows, is the presence of ovarian follicular cysts (FC) caused by hormonal factors. Nowadays do not exist reports that indicate some relation between the presence of these cysts and infectious agents, therefore the main objective of this study was to identify the association of FC with the presence of antibodies and antigens of reproductive diseases such as leptospirosis, brucellosis, neosporosis, bovine viral diarrhea, and infectious bovine rhinotracheitis in milk cows. One hundred and sixteen Holstein milk cows were selected and 4 groups of animals were formed. The group 1 was formed by 35 cows from milk production unit clinically healthy (UPH) and with more than two births each. The group 2 was formed by 28 cows from a milk production unit with ovarian follicular cysts (›to 2 cm of diameter) (UPFC). The group 3 was formed by 30 controls cows that were sent to slaughter without FC (SOH) and the group 4 was formed by 23 cows that were sent to slaughter with FC (SOC). The serum samples and follicular liquid (FL) of the cows of each group were analyzed for antibodies detection through recommended serologic techniques for each disease. For the detection of genetic material of the agents involved in each disease, different PCR protocols from FL samples of the animals with and without FC were applied. Additionally with the purpose of to corroborate the FC presence and to discard another type of ovarian cysts, the ovaries with cysts were processed by the histological routine technique. The results were analyzed by means of descriptive statistic. In order to determine if the presence of serum and LF antibodies of the animals differed with the presence of the microorganisms detected according to the animal group, nonparametric tests (Extreme of positions of Wilcoxon/U Mann-Whitney) with a degree of significance of 0.05 were used. Statistically was determined that there was no difference between the animal groups analyzed for each disease p › 0.05 with respect to the presence of serum antibodies (AbS), (p›0.05). Nevertheless, there was statistical difference of group 3 (SOH) with the rest of the groups respect to the presence of antibodies in follicular liquid (AbFL) for L. interrogans (p= 0,002) and Brucella sp. (p= 0,006). In addition in the four animal groups, it was determined statistically that there was difference between the animals with AbS respect to those with AbFL for Brucella sp. (p= 0,003) and the bovine viral diarrhea virus (BVDV) (p= 0,000), whereas for L. interrogans, Neospora caninum and the Infectious bovine rhinotracheitis virus (IBRV) there was no statistical difference (p› 0.05). In relation to animals that presented AbS and FC, statistically was found that they are independent for different diseases (p› 0.05). Whereas for animals that present AbFL and the manifestation of a cyst, it was found a possible relation of these elements for L. interrogans (p= 0.000*) and Brucella sp. (p= 0,004 **).However, there was no relation between the presence of AbFL for Neospora caninum, BVDV and IBRV and the presence of cysts (p› 0.05). With respect to the PCR results, only 4 samples for Brucella abortus and 14 samples for the IBRV were positive. According to the results of the present study, it is concluded that Brucella sp., Leptospira interrogans, Neospora caninum, BVDV and IBRV, are not related directly to the generation of follicular cysts.

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