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2011, Number 1

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Rev Cubana Plant Med 2011; 16 (1)

Anti-inflammatory and antimitotic effect of the alcoholic extract and chemical composition of the oil from Conyza bonariensis (L.) Cronquist (deer shinbone) leaves

Manzano SP, Miranda M, Gutiérrez Y, García G, Orellana T, Orellana-Manzano A

Language: Spanish
References: 18
Page: 13-23
PDF size: 191.19 Kb.


ABSTRACT

Introduction: the Conyza bonariensis (L). Cronquist species, usually called deer shinbone is of American origin.
Objectives: to contribute to the pharmacological and toxicological study of alcoholic extracts and to the chemical assessment of C. bonariensis leaf oil from Ecuador.
Methods: two 50 % tinctures of 70 % and 90 % ethanol content in dry leaves were used. A phytochemical study was conducted by an combined system of mass spectrometry and gas chromatography; the amniotic effect was determined using plate dilution inhibition with surface culturing by dissemination, and the antiinflammatory effect in the Croton oil induced ear edema model in OF1 line albino mice.
Results: the phytochemical screening showed the presence of essential oils, triterpene-steroids, phenolic compounds, flavonoids, quinones, anthocyanidines, saponins and reducing compounds. The structures of 32 essential oil components extracted from the leaves were identified along with and the sesquiterpene trans-β- farnesene that was the major component (70.82 %), this compound is reported for the first time in this species. The hydroalcoholic extract (25 µL on each side of the treated ears) significantly inhibited inflammation compared to benzidamine.
Conclusions: hydroalcoholic extracts showed anti-inflammatory effect in the experimental conditions of the study and the chemical composition of C. bonariensis leaf oil was identified.


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