Isea FGA, Emérita RI, Sánchez CEE, Montero UMA

Language: Spanish
References: 8
Page: 183-189
PDF size: 148.83 Kb.

ABSTRACT

Introduction: both Phyllanthus niruri L. and Azadirachta indica A. Juss. are used in Venezuela on an empirical basis for the treatment of diabetes mellitus. It was considered important to verify the potential usefulness to face acute hyperglicemic condition; and taken into account some adverse effects of A. indica, to value possible synergic effects with P. niruri that allow reducing the dose and taking advantage of its therapeutic pharmacological action.
Objectives: to assess the acute hypoglycemic or anti-hyperglycemic effect.
Methods: aqueous extracts from A. indica leaves and the P. niruri plant were prepared first, then 70 g of vegetal material were weighted and placed into 200 mL of water at 55-60 ºC. Three courses of treatment were organized; two included each extract and the third based on the combination of equal portions of them. Each extract and the combination were measured their content of glycose in order to prepare glycoside solutions of 13.77, 21.54 and 18.82 mmol/L, which acted as controls of the therapies with A. indica, P. niruri and their combination. They were orally administered at a dose of 5 mL/300 g and compared before and after the administration. Likewise, glycemia was measured before and 2 hours after treatment.
Results: in the comparison with its glycoside solution control, only the combination of extracts significantly reduced glycemia. As to the comparison before and after the administration, all the treatments managed to decrease in a significant way glycemia; however, in glycoside solution controls, there was not remarkable variation of glycemia.
Conclusions: there was proved the hypoglyceemic effect of the whole P. niruri plant, leading to its validation as a Venezuelan traditional medicinal plant and the synergic effect that may be achieved with A, indica leaves was put forward.

REFERENCES

1. Sokeng SD, Rokeya B, Mostafa M, Nahar M, Mosihuzzaman M, Ali L, et al. Antihyperglycemic effect of Bridelia ndellensis ethanol extract and fractions in streptozotocin-induced diabetic rats. African J Traditional Complementary Alternative Medicines. 2005;2(2):94-102.

2. Ebong PE, Atangwho IJ, Eyong EU, Egbung GE. The antidiabetic efficacy of combined extracts from two continetal plants: Azaridachta indica (A. Juss) (Neem) and Veronia amygdalina (Del.) (African Bitter Leaf). American J Biochemistry Biotechnology. 2008;4(3):239-44.

3. Hem A, Smith, AJ, Solberg P. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guineapig, ferret and mink. Laboratory Animals. 1998;32:364-8.

4. Eseyin OA, Ebong P, Ekpo A, Igboasoiyi A, Oforah E. Hypoglycemic effect of the seed extract of Telfairia occidentalis in rat. Pakistan J Biological Sciences. 2007;10(3):498-501.

5. Ministerio del Poder Popular para Ciencia, Tecnología e Industrias Intermedias. Fondo Nacional de Ciencia, Tecnología e Innovación. República Bolivariana de Venezuela. (2008). Código de Bioética y Bioseguridad. 3ra ed. Caracas. Disponible en: http://www.fonacit.gov.ve/documentos/bioetica2009.pdf

6. Gidado A, Ameh DA, Atawodi SE. Effect of Nauclea latifolia leaves aqueous extracts on blood glucosa levels of normal and alloxan-induced diabetic rats. African J Biotechnology. 2005;4(1):91-3.

7. Alarcón-Aguilar FJ, Banderas-Dorante T, Gutiérrez-León A, Vázquez-Carrillo L, Flóres-Saenz JL, Román-Ramos R. Study of the anti-hyperglycemic effect of antidiabetic plants in rabbits with impaired glucosa tolerante. Proceedings Western Pharmacology Society. 2003;46:148-52.

8. Patel VS, Chitra P, Prasanna PL, Krishnaraju V. Hypoglycemic effect of aqueous extract of Parthenium hysterophorus L. in normal and alloxan induced diabetic rats. Indian J Pharmacology. 2008;40(4):183-5.