Pérez FMG, Castrejón VMI, Vargas CME, Guido BRL, Ortiz CF, Galicia TJ, Otero MLG

Language: Spanish
References: 25
Page: 228-234
PDF size: 427.70 Kb.

ABSTRACT

Background: Clinical diagnosis has undergone several modifications due manly to the advances generated by the new methods of cellular analysis. Flow cytometry identifies different lymphocytes subsets for the diagnostic and evaluation of a great variety of illnesses. For that, it is necessary to determine the variation and reference values in healthy subjects.
Objective: To know reference values for CD3, CD4, CD8 and CD4/CD8 analyzing values of lymphocytes subsets in healthy donors by flow cytometry.
Material and method: A descriptive, cross-sectional and comparative study was done with 51 healthy adults (30 male and 21 female) of 18 to 57 years old from Central Blood Bank of National Medical Center 20 de Noviembre, Mexico City, were selected. The determination of subsets of lymphocytes T CD4+ and T CD8+ was carried out by lysis non laundry with flow cytometry FACSCalibur of Becton Dickinson.
Results: Results and reference intervals obtained for the total group were: for CD3 a media of 194, interval of 852-3,264, with p (‹1) 0.78; for CD4, a media of 1,145, interval of 580-1,944 and p (‹1) 0.40; for CD8, a media of 771, interval of 264-1,533 and p (‹ 0.05) 0.10; for the relationship CD4/CD8, a media of 1.67, interval of 0.59-3.1 and p (‹1) 0.063.
Conclusions: Data obtained were correlated with previous values used in hystocompatibility laboratory, and significant differences were observed. It is necessary to perform more extensive research in order to compare results.

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