medigraphic.com

2013, Number 4

Finlay 2013; 3 (4)

Reference ranges for serum T3 and T4 concentrations. A preliminary study

Miranda PC, Miranda PCA, Quintana CD

Language: Español
References: 10
Page: 230-234
PDF size: 110.35 Kb.


ABSTRACT

Background: quantitative determination of thyroid hormones (T3 and T4) is of great importance for the diagnosis and assessment of hyperthyroidism, especially isolated hyperthyroidism caused by T3.
Objective: to establish reference ranges for T3 and T4 in the nuclear medicine laboratory of the Dr. Gustavo Aldereguía Lima University General Hospital in Cienfuegos.
Methods: a prospective descriptive study was conducted using the radioimmunoassay method, a competition between unlabeled T3 and T4 and the hormone labeled by a limited number of binding sites on the specific antibody. By the reaction between a fixed amount of tracer and antibody with different amounts of unlabeled ligand, the amount of tracer bound by the antibody will be inversely proportional to the concentration of unlabeled ligand.
Results: the values obtained are described with a Gaussian distribution (arithmetic mean = 117, SD = 31 for T4; arithmetic mean = 2.64, SD = 0.64 for T3), which was confirmed by a Chi-square test. Normal value ranges were 55-178 nmol/L and 1.4-3.9 nmol/L for T4 and T3, respectively.
Conclusion: reference ranges obtained were larger than those proposed by the producer, especially in the case of T4.

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