Ramírez AMÁ, Zamora PAL, Arámbula GR, Ortiz GY, Guerrero VC, Martínez RV, Gómez MBC, Zúñiga GG

Language: Spanish
References: 14
Page: 60-66
PDF size: 326.19 Kb.

ABSTRACT

Background: Micronucleus assay detects DNA damage. Periodontitis is a chronic degenerative disease of the oral cavity and the generation of free radicals is an important consideration in this disease that could result in an increased frequency of DNA damage. There is evidence in the literature suggesting an association between periodontal diseases in pregnant women and giving birth to premature infants. Also we have investigated whether pathologies that are associated with the formation of free radicals lead to an increase the frequency of micronucleus in preterm newborns during pregnancy. Objective: Evaluate DNA damage increased by means of micronuclei assay in buccal mucosa cells from puerperal women with periodontitis and to determinate the teratogenic potential in the preterm newborns to mothers with periodontitis by means of counting micronucleus in peripheral blood. Material and methods: Buccal mucosa cells from puerperal woman with periodontitis and without periodontitis and peripheral blood from preterm newborns were collected and spread directly on two separate, pre-cleaned and pre-coded slides. The smears were air-dried, fixed, stained and analyzed by microscopy. DNA damage was determined by counting micronucleus and nuclear abnormalities in exfoliated cells, and in peripheral blood the number of micronucleated erythrocytes, micronucleated polychromatic erythrocytes and the number of PCE. Conclusion: The increase in micronucleus and nuclear abnormalities frequency in buccal mucosa cells of puerperal women with periodontitis and the increased in micronucleated polychromatic erythrocytes number in preterm newborns to mothers with periodontitis indicates that the periodontitis per se increases DNA damage and its potential teratogenic effect on preterm newborns to mothers with periodontitis.

REFERENCES

1. Canakci CF, Cicek Y, Yildirim A, Sezer U, Canakci V. Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. Eur J Dent. 2009; 3: 100-106.

2. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999; 4: 1-6.

3. Zamora-Pérez AL, Lazalde-Ramos BP, Sosa-Macías MG, Gómez-Meda BC, Torres-Bugarín O, Zúñiga-González GM. Methylphenidate lacks genotoxic effects in mouse peripheral blood erythrocytes. Drug Chem Toxicol. 2011; 34: 294-299.

4. Chang TI, Loeken MR. Genotoxicity and diabetic embryopathy: impaired expression of developmental control genes as a cause of defective morphogenesis. Semin Reprod Endocrinol. 1999; 17: 153-165.

5. Batista-González CM, Corona-Rivera JR, Gómez-Meda BC, Zamora-Pérez AL, Ramos-Ibarra ML, Zúñiga-González GM. Micronucleated erythrocytes in preterm newborns in relation to maternal pathology. Rev Biomed. 2006; 17: 11-16.

6. Zúñiga-González G, Gómez-Meda BC, Zamora-Pérez A, Ramos-Ibarra ML, Batista-González CM, Espinoza-Jiménez S, Gallegos-Arreola MP, Álvarez-Moya C, Torres-Bugarín O. Induction of micronuclei in proestrus vaginal cells from colchicine-and cyclophosphamide-treated rats. Environ Mol Mutagen. 2003; 42: 306-310.

7. Ceppi M, Biasotti B, Fenech M, Bonassi S. Human population studies with the exfoliated buccal micronucleus assay: Statistical and epidemiological issues. Mutat Res. 2010; 705: 11-19.

8. Migliore L, Botto N, Scarpatto R, Petrozzi L, Cipriani G, Bonuccelli U. Preferential occurrence of chromosome 21 malsegregation in peripheral blood lymphocytes of Alzheimer disease patients. Cytogenet Cell Genet. 1999; 87: 41-46.

9. Ramos-Remus C, Dorazco-Barragan G, Aceves-Avila FJ, Alcaraz-Lopez F, Fuentes-Ramirez F, Michel-Diaz J, Torres Bugarin O, Ventura-Aguilar A, Zuñiga-Gonzalez G. Genotoxicity assesment using micronuclei assay in rheumatoid arthritis patients. Clin Exp Rheumatol. 2002; 20: 208-212.

10. Torres-Bugarín O, Ventura-Aguilar A, Zamora-Perez A, Gómez-Meda BC, Ramos-Ibarra ML, Morgan-Villela G, Gutiérrez-Franco, Zúñiga-González G. Evaluation of cisplatin + 5-FU, carboplatin + 5-FU, and ifosfamide + epirubicine regimens using the micronuclei test and nuclear abnormalities in the buccal mucosa. Mutat Res. 2004; 565: 91-101.

11. Zúñiga-González GM, Batista-González CM, Gómez-Meda BC, Ramos-Ibarra ML, Zamora-Perez AL, Muñoz-Magallanes T, Ramos-Valdés C, Gallegos-Arreola MP. Micronuclei in diabetes: folate supplementation diminishes micronuclei in diabetic patients but not in an animal model. Mutat Res. 2007; 634: 126-134.

12. Nelly AL, Holford TR, Löe H, Änerud Ä, Boysen H. The natural history of periodontal disease in man. Risk factors for progression of attachment loss in individual receiving no oral health care. J Periodontol. 2001; 72: 1006-1015.

13. Lindhe J. Periodontología clínica e implantología odontológica. 5a ed. Argentina: Editorial Médica Panamericana; 2005: 430.

14. Gómez-Meda BC, Zúñiga-González GM, Zamora-Pérez A, Ramos-Ibarra ML, Batista-González CM, Torres-Mendoza BM. Folate supplementation of cyclophosphamide-treated mothers diminishes micronucleated erythrocytes in peripheral blood of newborn rats. Environ Mol Mutagen. 2004; 44: 174-178.