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2013, Number 3

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Biotecnol Apl 2013; 30 (3)

Molecular characterization of the Cuban Siboney cattle

Uffo O, Acosta A, Ribot A, Ruiz K, Ronda R, Martínez S
Full text How to cite this article

Language: English
References: 8
Page: 232-233
PDF size: 80.80 Kb.


Key words:

Cuban Siboney cattle, molecular markers, marker-assisted selection, microsatellite.

ABSTRACT

The new genotype of the Cuban Siboney cattle (5/8H×3/8C) was obtained as part of the genetic improvement strategy to obtain cattle with best fitted behavior under the Cuban climatic conditions and adapted to the tropics. Until 2002, its genetic background had been evaluated based on genetic parameters (progeny and behavior tests, production, and butyric fat yields). The results of the molecular characterization of the Cuban Siboney cattle are presented, from breeds located at the Cuban western regions. The genotypes of cattle six major milk proteins were determined and associative studies to production characters were initiated. RAPD and microsatellite markers were also typified for biodiversity studies. Gene frequencies were obtained for the bovine growth hormone, prolactin and their receptors, and also stearyl-coenzyme A desaturase (SCD) and the diacylglycerol acyltransferase 1 (DAGT1), these two last genes involved in lipids metabolism as candidates for marker-assisted selection. It was demonstrated that the Cuban Siboney cattle has the genetic potential to persist as prominent breed for milk production purposes in the nationwide program of genetic improvement, with high genetic variability and its own molecular characteristics. These results can be used as tools to increase the selection efficiency and to provide greater knowledge on the studied breed. This research won the 2012 Award of the Cuban National Academy of Sciences.


REFERENCES

  1. López D. Cruzamientos en Cuba: Experiencias y perspectivas. 1998. Available from: http://www.alpa.org.ve/PDF/ publica/CAP%204.pdf

  2. Suárez MA, Zubizarreta I, Pérez T. Interacción genotipo ambiente en ganado bovino Siboney de Cuba. Livestock Res Rural Dev. 2003;21(9):139.

  3. Uffo O, Martín-Burriel I, Martínez S, Ronda R, Osta R, Rodellar C, et al. Caracterización genética de seis proteínas lácteas en tres razas bovinas cubanas. Anim Genet Resour. 2006;39:15-24.

  4. Acosta A, Sanz A, Uffo O, Ronda R, Osta R, Martin-Burriel I, et al. Development of an eigth-plex SNP assay associated with milk production. Arch Zootec. 2011;60(231):595-606.

  5. Acosta A, Sanz A, Ronda R, Osta R, Rodellar C, Martin-Burriel I, et al. Proteínas lácteas en poblaciones bovinas cubanas. Comparación de la frecuencia alélica de las proteínas lácteas en cinco poblaciones bovinas cubanas. Anim Genet Resour. 2012;51:131-7.

  6. Acosta A, Uffo, O, Vazquez S, Salazar E. Estructura genética de tres poblaciones de ganado bovino autóctono cubano. Rev INFOVET. 2010;(mayo):23-4.

  7. Acosta A, Uffo O, Sanz A, Ronda R, Osta R, Rodellar C, et al. Genetic diversity and differentiation of fi ve Cuban cattle breeds using 30 microsatellite loci. J Anim Breed Genet. 2012;130(1)79-86.

  8. Acosta A, Ronda R, López F, Fernandes Z, Gomes-Filho MA, Uffo O, et al. Polimorfi smo genético en los receptores de la hormona del crecimiento y prolactina en el Siboney de Cuba. Desarrollo de metodologías. Rev Salud Anim. 2012; 34(2):109-14.




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Biotecnol Apl. 2013;30