Investigación en Discapacidad

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>Journals >Investigación en Discapacidad >Year 2014, Issue 1

López-Jácome LE, Hernández-Durán M, Colín-Castro CA, Ortega-Peña S, Cerón-González G, Franco-Cendejas R
Basic stains in the microbiology laboratory
Investigación en Discapacidad 2014; 3 (1)

Language: Español
References: 32
Page: 10-18
PDF: 389.70 Kb.

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Stains in microbiology lab are the first tools employed for diagnosis of infectious diseases. Stains have helped to identify the ethiology of microbial involvement for more than a century. As the wide spectrum of stains is currently in progress for the detection of bacteria, fungus and parasites, such fundamentals as the Gram stain are still considered basic for initial evaluation of bacteriological samples, while the Wright stain is used for very particular diseases as those related to parasites. Other specific tintorial methods as the Ziehl-Neelsen stain are used for diagnosis of chronic diseases such as tuberculosis oractinomycosis. The lactophenol blue stain is best employed for diagnosis of fungal infections, as it helps to identify fungi and to preserve their morphological structures. Different stains in the clinical microbiological lab are permanent basic tools for the diagnosis and treatment of many diseases of infectious ethiology.

Key words: Laboratory stains, microorganism, microbiology, Gram, Wright, Ziehl-Neelsen, lactophenol blue.


  1. 1.Decré D, Barbut F, Petit JC. Role of the microbiology laboratory in the diagnosis of nosocomial diarrhea. Pathol Biol (París). 2000; 48: 733-744.

  2. 2.Keller PJ. Imaging morphogenesis: technological advances and biological insights. Science. 2013; 340: 123-168.

  3. 3.Madison BM. Application of stains in clinical microbiology. Biotech Histochem. 2001; 76: 119-125.

  4. 4.Luis SBM, Altava B. Introducción a la química orgánica. Universitat Jaume;1997.

  5. 5.Fung DC, Theriot JA. Imaging techniques in microbiology. Curr Opin Microbiol. 1998; 1: 346-351.

  6. 6.Ronay V, Attin T. Black stain –a review. Oral Health Prev Dent. 2011; 9: 37-45.

  7. 7.Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev. 2011; 24: 247-280.

  8. 8.Calvo GA, Esteban RFJ, Montuenga FL. Técnicas en histología y biología celular. 2nd ed. Madrid Spain: Elsevier; 2009.

  9. 9.Clodfelter RL Jr. The peripheral smear. Emerg Med Clin North Am. 1986; 4: 59-74.

  10. 10.Beveridge TJ. Use of the Gram stain in microbiology. Biotechnic & Histochemistry. 2001; 76: 111-118.

  11. 11.Kaplan ML, Kaplan L. The Gram stain and differential staining. J Bacteriol. 1933; 25: 309-321.

  12. 12.Nagata K, Mino H, Yoshida S. Usefulness and limit of Gram staining smear examination. Rinsho Byori. 2010; 58: 490-497.

  13. 13.Breakwell DP, Moyes RB, Reynolds J. Differential staining of bacteria: capsule stain. Curr Protoc Microbiol. 2009. Appendix 3: p. Appendix 3I.

  14. 14.Beveridge TJ, Graham LL. Surface layers of bacteria. Microbiol Rev. 1991; 55: 684-705.

  15. 15.Coico R. Gram staining. Curr Protoc Immunol. 2001. Appendix 3: p. Appendix 3O.

  16. 16.Popescu A, Doyle RJ. The Gram stain after more than a century. Biotech Histochem. 1996; 71: 145-151.

  17. 17.Beveridge TJ. Mechanism of Gram variability in select bacteria. J Bacteriol. 1990; 172: 1609-1620.

  18. 18.Murray P. Manual of clinical microbiology. 9th ed. USA: American Society for Microbiology; 2007.

  19. 19.Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998; 62: 1094-1156.

  20. 20.Koneman E. Diagnóstico microbiológico. 6a ed. México DF: Editorial Médica Panamericana S.A.; 2006.

  21. 21.Forbes BA, Sahm D, Weissfeld A. Bailey & Scott. Diagnóstico microbiológico. 12a ed. Editorial Médica Panamericana S.A.; 2009.

  22. 22.Ben-Selma W et al. Rapid detection of Mycobacterium tuberculosis in sputum by Patho-TB kit in comparison with direct microscopy and culture. Diagn Microbiol Infect Dis. 2009; 65: 232-235.

  23. 23.Chen P et al. A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. J Clin Microbiol. 2012; 50: 1166-1170.

  24. 24.Selvakumar N et al. Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli. J Clin Microbiol. 2002; 40: 3041-3043.

  25. 25.Prescott LM. Microbiology. 5th ed. USA: McGraw-Hill; 2002.

  26. 26.Selvakumar N et al. Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli. Int J Tuberc Lung Dis. 2005; 9: 226-229.

  27. 27.Salud OPDL. Manual para el diagnóstico bacteriológico de la tuberculosis. Vol. 1. 2008.

  28. 28.Arredondo GCJ. Conceptos clínicos de infectología. 10a ed. México DF: Méndez Editores;1995.

  29. 29.Farrant JL. An electron microscopic study of ferritin. Biochim Biophys Acta. 1954; 13: 569-576.

  30. 30.Ohi M et al. Negative staining and image classification - powerful tools in modern electron microscopy. Biol Proced Online. 2004; 6: 23-34.

  31. 31.Mc KR. Staining bacterial polysaccharides. J Bacteriol. 1953; 66: 453-454.

  32. 32.Larone DH. Medical important fungi. A guide to identification. 5th ed. USA: American Society for Microbiology; 2011.

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