Sánchez FP, Cuellar CY, Sánchez BMJ, Oropesa CM, Reinaldo MK, Collazo GS

Language: Spanish
References: 10
Page: 241-245
PDF size: 148.65 Kb.

ABSTRACT

Introduction: Reagent red cells are used in blood banks and transfusion services as analytical control before a negative result of the human antiglobulin test. Their use is not universal due, fundamentally, to the insufficient availability of commercial cells and the short life span of the cells prepared by the laboratory. Objective: To develop a methodology for the preparation of checks cells based on traditional methods. Material and methods: We used erythrocytres O of a known phenotype from a regular cell donor and hyperimmune anti D plasma with antibody titer 1/32 of a regular plasma donor by apheresis production. A weakly washed erythrocyte suspension was prepared, sensitized following established procedures, using SAGM solution as preservative. Physicochemical and immunohematologic parameters were evaluated immediately after the preparation and weekly to record their stability. Results: The check cells prepared proved to be functionally suitable for their use in vitro as quality control in the Coombs test, with a 30-day life span. The method is appropriate for the initial purposes as it provides check cells without the need of prior treatment, or frequent recourse to the donor, its preparation being simple and economical.

REFERENCES

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