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Revista ADM Órgano Oficial de la Asociación Dental Mexicana

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Órgano Oficial de la Asociación Dental Mexicana
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2014, Number 5

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Rev ADM 2014; 71 (5)

Detection of Legionella pneumophila in the water supply systems at the Autonomous University of the State of Mexico’s (UAEM) Faculty of Dentistry

Manzanares LGL, Montiel BNM, del Real SSGME, Flores CRI
Full text How to cite this article

Language: Spanish
References: 14
Page: 216-220
PDF size: 209.75 Kb.


Key words:

Legionella pneumophila, biofilm, dental units.

ABSTRACT

Background: Water supply systems, including those of dental units, are prone to the development of biofilms that may be colonized by opportunistic pathogens such as Legionella pneumophila, a type of bacteria that can cause severe lung diseases. Objective: To determine, for the first time, whether Legionella pneumophila is present in the water supply systems of the Faculty of Dentistry at the UAEM. Material and methods: An observational, cross-sectional study was performed in which 120 water samples were collected from the Faculty of Dentistry of the UAEM, including from the water supply lines of dental units, the filters, and the publically-used faucets. These were then cultivated in BCYEα agar with L-cysteine and verified using blood agar. CFU/mL were counted and an ANOVA test and a Bonferroni test were applied with the help of SPSS.21 software package for statistical analysis. Results: 100% of the samples displayed growth in BCYEα agar and an absence in blood agar. There were statistically significant differences (p ≤ 0.05) in all of the tests. Conclusions: Six-monthly monitoring is recommended in order to allow timely corrections to be made and to avoid any increase in the bacteria and the development of disease.


REFERENCES

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Rev ADM. 2014;71