Validation of flavonoid quantification method in Passiflora incarnata L. tablets

Raúl Nápoles López; Jesús Rafael Rodríguez Amado; Maricel Rodríguez Antomachi; Ariadna Lafourcade Prada

2014, Number 1

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Rev Cubana Farm 2014; 48 (1)

**Validation of flavonoid quantification method in Passiflora incarnata L. tablets**

Nápoles LR, Rodríguez AJR, Rodríguez AM, Lafourcade PA

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Language: Spanish
References: 9
Page: 129-138
PDF size: 196.79 Kb.

ABSTRACT

Introduction: to validate the flavonoid quantification method on Passiflora incarnata L. tablets by using quercetine as standard.
Methods: selectivity, linearity, accuracy, precision and robustness were evaluated, using USP 30 and the regulations of the Center for the State Control of Drug Quality (CECMED).
Results: under the conditions of this study, the method is selective, linear in a range of 60 to 140 % of flavonoid concentration. It showed accuracy and precision with global relative standard deviation of 0.67 %. The results did not significantly vary when the analytical conditions changed.
Conclusions: under the established conditions, this method is reliable and allows obtaining appropriate results for the intended use.

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