Rincón ACM, Patiño LOJ, Plazas GEA, Bulla NME, Rozo TG, Puyana HM

Language: Spanish
References: 22
Page: 138-150
PDF size: 213.26 Kb.

ABSTRACT

Introduction: studies about the phytochemistry and biological properties of the species Pernettya prostrata (Cav.) DC. (Ericaceae) conducted so far have been preliminary and many of the results published are not totally reliable. Therefore, the plant is an interesting and promising object of study.
Objective:carry out the preliminary phytochemical analysis and evaluation of some biological properties of extracts and fractions of P. prostrata.
Methods: preliminary phytochemical analysis was conducted of ethanolic extracts from the aerial parts and fruits for determination of possible constituents. Antioxidant activity was evaluated by DPPH free radical capture. Toxic activity against Artemia salina and antifeedant activity against Sitophilus zeamais and Tribolium castaneum were also determined.
Results: preliminary phytochemical tests suggest the presence of flavonoids, tannins, triterpenes and/or steroids in ethanolic extracts from fruits and aerial parts of P. prostrata. Squalene and β-1-O-methoxy-glucopyranose were isolated and identified from the phytochemical study of the fruit ethanolic extract. The compounds isolated and identified did not reveal any promising results in the biological assays performed. Ethanolic extracts from fruits (72.8 ppm) and aerial parts (60.9 ppm) exhibited similar antioxidant activity. The methanolic fraction showed the highest antioxidant activity with IC 50 (86.2 ± 8.8 ppm) and IP (89.8 %), greater than those in the ascorbic acid used as a standard (IC50 = 49.3 ± 0.2 ppm). No toxic or antifeedant activity was found in the extracts and fractions evaluated.
Conclusions: the present paper makes a contribution to knowledge about the chemical composition and biological properties of the species P. prostrata through isolation and identification of two new substances, and determination of the antioxidant activity of some extracts and fractions.

REFERENCES

1. Salinas NR y Betancur J. Las ericáceas de la vertiente pacífica de Nariño, Colombia (1ra ed.). Bogotá: Instituto de Ciencias Naturales e Instituto de Investigación de Recursos Biológicos Alexander von Humboldt; 2005. pp. 12-29.

2. Jun Li, Fu Li, Yuan-Yuan L, Xiao-Jian S, Cui-Ping H, Xue-Wen L. A new dilactone from the seeds of Gaultheria yunnanensis. Fitoterapia. 2010;81(1):35-7.

3. Li-Quan W, Guo-Wei Q, Gang L, Kin-Fai C. Grayanoids from Pieris formosa. Phytochemistry. 1998;49(7):2045-8.

4. Li-Quan W, Guo-Wei Q, Shao-Nong Chen, Can-Jun Li. Three diterpene glucosides and a diphenylamine derivative from Pieris formosa. Fitoterapia. 2001;72(7):779- 87.

5. Yuhua C, Qingcui C, Jiannong Y. Chromatographic and electrophoretic methods for pharmaceutically active compounds in Rhododendron dauricum. Journal of Chromatography B. 2004;812(1-2):231-40.

6. Schultes R. The botanical and chemical distribution of hallucinogens. Annu. Rev. Plant. Physiol. 1970;21:571-98.

7. Shigeki H, Iwao M, Masaru K, Orlando M y Mariano C. Sesquiterpenes from Pernetty afurens. Phytochemistry. 1985;24(10):2317-23.

8. Correa J, Bernal H. Especies vegetales promisorias de los países del convenio Andrés Bello. Tomo 7. Bogotá: Convenio Andrés Bello en coedición con la Junta de Acuerdo de Cartagena, la Corporación Andina de Fomento y el Ministerio de Educación y Ciencia de España; 1992. pp. 156-180.

9. García H. Flora Medicinal de Colombia, Botánica Médica. (3ra ed). Bogotá: Tercer Mundo Ediciones; 1992. pp.165-179.

10. Vicente T, Omar M, Paola V, Giovanni V, Chabaco A, Tomás Z. An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador. Journal of Ethnopharmacology. 2007;111(1):63-81.

11. Sanabria A. Colección de especies vegetales y análisis fitoquímico preliminar. Bogotá: Facultad de Ciencias, Universidad Nacional de Colombia; 1999. pp.134- 190.

12. Lock de Ugaz O. Investigación fitoquímica. Métodos en el estudio de productos naturales. Perú: Pontificia Universidad Católica; 1988. pp.73-150. Revista Cubana de Plantas Medicinales 2014;19(2):138-150 http://scielo.sld.cu 150

13. Jing D, Wangyuan C, Guangzhong Y. A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chemistry. 2011;125(4):1430-5.

14. Argolo AC, Sant'Ana AE, Pletsch M, Coelho LC. Antioxidant activity of leaf extracts from Bauhinia monandra. Bioresource Technology. 2004; 95(2):229-33.

15. Mc Laughlin J, Rogers L, Anderson J. The use of biological assays to evaluate botanicals. Drug Information Journal. 1998; 32(2):513-24.

16. Ticona V, Nieta M, Irahola P, Gimenez A. Pruebas biológicas destinadas a evaluar citotóxicos como indicadores de potenciales antitumorales. Biofarbo. 1998: 6(6):11-16.

17. Xie Y, Bodnaryk R. A rapid and simple flour-disk bioassay for testing substances active against stored-product insects. The Canadian Entomologist. 1996;128(5):865-75.

18. Liua Z, Goh S, Hob S. Screening of Chinese medicinal herbs for bioactivity against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Journal of Stored Products Research. 2007; 43(3):290-296.

19. Häkkinen S, Kärenlampi S, Heinonen I, Mykkänen HM, Törrönen AR. Content of the Flavonols Quercetin, Myricetin, and Kaempferol in 25 Edible Berries. Journal of Agricultural and Food Chemistry. 1999;47(6):2274-9.

20. Soler-Rivas C, Espín JC, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochemical Analysis. 2000;11(5):330-8.

21. Khurshid A, Aziz AR, Abdullahil B, Golam S. Chemical constituents of Hemigraphishirta T. Anders (Acanthaceae). Pakistan Journal of Biological Sciences. 2002;5(11):1264-6.

22. Mattias U, Petri T, Matthias N, Rainer S. Complete assignments of the 1H and 13C chemical shifts and JH, H coupling constants in NMR spectra of Dglucopyranose and all D-glucopyranosyl-D-glucopyranosides. Carbohydrate Research. 2008;343(1):101-12.