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2014, Number 4

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Rev Cubana Pediatr 2014; 86 (4)

Interleukin-6 and tumor necrosis factor-α in serum and cerebrospinal fluid from children with viral meningoencephalitis

Luis ÁMC, Alonso CA, González AM, Araña RM, Sarmiento PL

Language: Spanish
References: 22
Page: 445-453
PDF size: 201.54 Kb.


ABSTRACT

Introduction: viral meningoencephalitis affecting the child is caused mainly by enteroviruses and have generally self-limited benign course.
Objective: to estimate the interleukin-6 and tumor necrosis factor-α present in the serum and the cerebrospinal fluid of children suffering viral meningoencephalitis.
Method: cross-sectional descriptive study of 61 patients aged one month to 17 years and diagnosed with viral meningoencephalitis. The diagnosis was based on their clinical picture, the cytochemical study of their cerebrospinal fluid and the detection of enterovirus viral genome through the reverse transcriptase polymerase chain reaction. Forty eight serum samples and 35 cerebrospinal fluid samples served to determine the interleukin-6 and tumor necrosis factor-α concentrations. The statistical analysis included Wilcoxon's test for 2 related samples and the Spearman's test for correlating cytokine concentrations.
Results: viral genome was detected in 3 cerebrospinal fluid samples with increased cellularity and predominant polymorphonuclear neutrophils and in other 3 samples with normal cellularity. The quantitation of serum interleukin-6 and of tumor necrosis factor-alpha were 91.6 ± 39.0 pg/mL and 106.5 ± 54.6 pg/mL, respectively. The interleukin-6 and tumor necrosis factor-alpha levels in the cerebrospinal fluid amounted to 109.65 ± 49.6 pg/mL, and 75.28 ± 29.9 pg/mL, respectively.
Conclusions: high interleukin-6 and tumor necrosis factor-alpha levels were found in all the studied samples. The factor concentrations were higher in the serum whereas those of interleukin-6 were higher in the cerebrospinal fluid; there was positive correlation between interleukin-6 values in the serum and those of the tumor necrosis factor-alpha.


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