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2015, Number 1

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Medicentro 2015; 19 (1)

Evaluation of hypolipidemic potential of Cymbopogon citratus S. in a model of acute hyperlipidemia

Betancourt ME, González MY, Escobar RR, Bermúdez TD, Blanco MF, Martínez MCM

Language: Spanish
References: 23
Page: 2-12
PDF size: 222.22 Kb.


ABSTRACT

A pharmacological study was conducted at the Department of Experimental Researches of the Experimental Toxicology Unit from Villa Clara, to evaluate the lipid-lowering effect of the species Cymbopogon citrates S. (lemongrass) in a model of acute hyperlipidemia induced by poloxamer 407, non-ionic detergent. C57BL/6J male mice were used; the extract was evaluated in doses of 400mg/kg and 600mg/kg (group V and VI respectively). Six experimental groups were formed: group I corresponded to the negative control, II to control group of hyperlipidemia, III and IV to control groups of sinvastatin and nicotinic acid, as well as, group V and VI corresponded to the evaluated doses of Cymbopogon citratus S extract. Phytochemical characterization of hydroalcoholic extract was done; there were also evaluated clinical signs of toxicity, the plasma concentration of total cholesterol, triacylglycerol (TAG) and very low- density lipoprotein (VLDL). The phytochemical screening confirmed the presence of reducing compounds, alkaloids and tannins, showing great concentration of flavonoids and triterpenes. Cholesterol, TAG and VLDL variables for groups V and VI showed significant differences with respect to control group of hyperlipidemia (II), and behaved similarly to the control groups of sinvastatin (III) and nicotinic acid (IV). As a conclusion, the Cymbopogon citrates S. extract at the doses studied in the model of acute hyperlipidemia presents hypolipidemic activity.


REFERENCES

  1. Chapman MJ. Pitavastatin: novel effects on lipid parameters. Atheroscler Suppl. 2011 Nov.;12(3):277- 84.

  2. Perk J, De Backer G, Gohlke H, Graham I, Reiner Ž, Verschuren M. et al. European Guidelines on cardiovascular disease prevention in clinical practice (version 2012) The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice. Eur Heart J [internet]. 2012 [citado 18 jun. 2013];33(13):[aprox. 66 p.]. Disponible en: http://eurheartj.oxfordjournals.org/content/33/13/1635

  3. Chitra Devi R, Sim SM, Ismail R. Effect of Cymbopogon citratus and Citral on Vascular Smooth Muscle of the Isolated Thoracic Rat Aorta. Evid Based Complement Altern Med [internet]. 2012 [citado 22 mar. 2014];2012:[aprox. 8 p.]. Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364612/pdf/ECAM2012-539475.pdf

  4. Bermúdez Toledo D, Escobar Román R, Boffill Cárdenas M, Betancourt Morgado E, Igualada Correa I. Evaluación del potencial hepatoprotector de la Mentha piperita L previo a la inducción de hepatotoxicidad con acetaminofén. Bol Latinoam Caribe Plant Med Aromat [internet]. 2014 nov. [citado 30 nov. 2014];13(6):[aprox. 12 p.]. Disponible en: http://www.blacpma.usach.cl/images/docs/013-006/006_articulo_5.pdf

  5. Rodríguez Quintanilla R, Ruiz Nova C, Arias Moyano G, Castro Salazar H, Martínez J, Stashenko E. Estudio comparativo de la composición de los aceites esenciales de cuatro especies del género Cymbopogon (Poaceae) cultivadas en Colombia. Bol Latinoam Caribe Plant Med Aromat [internet]. 2012 [citado 14 jun. 2013];11(1):[aprox. 9 p.]. Disponible en: http://www.blacpma.usach.cl/images/docs/011-001/013_elena.pdf

  6. Alvis A, Martínez W, Arrazola G. Obtención de extractos hidro-alcohólicos de limoncillo (Cymbopogon citratus) como antioxidante natural. Inform Tecnol. 2012;23(2):3- 10.

  7. He WS, Jia CS, Yang YB, Ma Y, Zhang XM, Feng B, et al. Cholesterol-lowering effects of plant steryl and stanyllaurate by oral administration in mice. J Agric Food Chem. 2011;59(9):5093- 9.

  8. Ministerio de Salud Pública. NRSP 310. Norma Ramal. Medicamentos de origen vegetal: Droga cruda. Especificaciones Generales. La Habana: MINSAP; 1992.

  9. Miranda M, Cuéllar A. Manual de Prácticas del Laboratorio de Farmacognosia y Productos Naturales. Instituto de Farmacia y Alimentos. La Habana: Pueblo y Educación; 2000. p. 53- 66.

  10. American Veterinary Medical Association. AVMA Guidelines for the Euthanasia of Animals: 2013 Edition [internet]. United States of America: Washington University, St. Louis Missouri; 2013 [citado 16 oct. 2013]. Disponible en: https://www.avma.org/kb/policies/documents/euthanasia.pdf

  11. Yasuda T, Johnston TP, Shinohara M, Inoue M, Ishida T. The effect of poloxamer 407 on the functional properties of HDL in mice. J Pharm Pharmacol. 2012;64(5):677- 87.

  12. Johnston TP. The P-407-induced murine model of dose-controlled hyperlipidemia and atherosclerosis: a review of findings to date. J Cardiovasc Pharmacol. 2004 Apr.;43(4):595- 606.

  13. Samer M, Fugen A, Neal M, Daviesc Basil D. Phytopreventative Anti-Hyperlipidemic Effects of Gynostemma Pentaphyllum in Rats. Pharm Pharmaceut Sci. 2005;8(3):507- 15.

  14. Ward S, Jones ML, Pandor A, Holmes M, Ara R, Ryan A, et al. A systematic review and economic evaluation of statins for the prevention of coronary events. Health Technol Assess. 2007;11(14):1- 160.

  15. Nuñez Cortez JM, Alegría E, Álvarez-Sala Walther L, Ascaso Gimilio J, Lahoz Rallo C, Mantilla Morató T, et al. Documento Abordaje de la dislipidemia. Sociedad Española de Arteriosclerosis (Parte II). Clín Invest Arterioscl [internet]. 2012 [citado 12 jun. 2013];24(1):[aprox. 13 p.]. Disponible en: http://www.searteriosclerosis.org/resources/archivosbd/clinica_documentos_guias/e2178056a5 01b24527a72965af54f965.pdf

  16. Al-Reza S, Bajpai V, Kang.S. Antioxidant and antilisterial effect of seed essential oil and organic extracts from Zizyphus jujube. Food Chem Toxicol. 2009;47:2374- 80.

  17. Cottyn B, Kollmann A, Waffo-Teguo P, Ducrot PH. Rationalization and In Vitro Modeling of the Chemical Mechanisms of the Enzymatic Oxidation of Phenolic Compounds in Planta: From Flavonols and Stilbenoids to Lignins. Chemistry. 2011;17(26):7282- 7.

  18. Kviecinski MR, Bettega Felipe K, Gomes Correia JF, Ferreira EA, Rossi MH, de Moura Gatti F, et al. Brazilian Bidens pilosa Linné yields fraction containing quercetin-derived flavonoid with free radical scavenger activity and hepatoprotective effects. Libyan J Med [internet]. 2011 [citado 12 jun. 2013];6(10):[aprox. 8 p.]. Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3081874/pdf/LJM-6-5651.pdf

  19. González Gil A, González Madariaga Y, Heredia Ruiz D, Fernández Caraballo D, Ballesteros Hernández M. Enzimas antioxidantes en la hiperglicemia e hiperlipidemia inducida por sacarosa en ratas Wistar. Rev Méd Electrón [internet]. 2013 mar.- abr. [citado 4 mayo 2013];35(2):[aprox. 10 p.]. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1684-18242013000200001&lng=es

  20. Sáyago Ayerdi SG, Goñi I. Hibiscus sabdariffa L: Fuente de fibra antioxidante. Arch Latinoam Nutr [internet]. 2010 mar. [citado 10 feb. 2013];60(1):[aprox. 6 p.]. Disponible en: http://www.scielo.org.ve/pdf/alan/v60n1/art12.pdf

  21. Lia RW, Theriaulta AG, Aub K, Douglasa TD, Casaschia A, Kurowskab EM, et al. Citrus polymethoxylated flavones improve lipid and glucose homeostasis and modulate adipocytokines in fructose-induced insulin resistant hamsters. Life Sci. 2006;79(4):365- 73.

  22. Green CO, Wheatley AO, Mcgrowder DA, Dilworth LL, Asemota HN. Hypolipidemic effects of ortanique peel polymethoxylated flavones in rats with diet-induced hypercholesterolemia. J Food Biochem. 2011;35(5):1555- 60.

  23. Ronceros G, Ramos W, Arroyo J, Galarza C, Ericson L, Gutiérrez AG, et al. Estudio comparativo del maíz morado (Zea mays L.) y simvastatina en la reducción de lípidos séricos de pacientes diabéticos normotensos con dislipidemia. An Fac Med [internet]. 2012 [citado 12 jun. 2013];73(2):[aprox. 5 p.]. Disponible en: http://www.scielo.org.pe/pdf/afm/v73n2/a06v73n2.pdf




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