2014, Number 2
Rev Cubana Farm 2014; 48 (2)
In vitro antiviral activity of aqueous extract from red seaweed Tricleocarpa fragilis to influenza A virus
Pérez-Riverol A, Piñon RA, Morier DLF, Acosta HB, Valdés IO, del Barrio AG
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influenza viruses are major human pathogens that cause over 500 000 deaths every year. The emergence of viral variants resistant to approved
antiviral drugs has prompted the search for new anti-influenza compounds. Alga could be used as a source for the development of new anti-influenza drugs, taking into account the diversity of secondary metabolites previously described in these organisms.
to evaluate the in vitro
antiviral activity of aqueous extract of the red
seaweed Tricleocarpa fragilis
against influenza A virus.
the mean citotoxicity (CC50
) concentration of the extract was determined by using the MTT reduction assay in MDCK cells. The mean inhibitory concentration (CI50
) was estimated by means of viral protein (hemagglutinin) test and a cytopathic effect inhibition test in MDCK cells. The Selective index was calculated from (SI)= CC50
Results: T. fragilis
was not toxic at the concentrations evaluated in MDCK cells. The aqueous extract inhibited in vitro influenza A(H1N1) and A(H3N2) virus replication with SI values › 11.4 and 106; respectively.
aqueous extract of T. fragilis
showed in vitro
anti-influenza activity and can be employed as a source for new antiviral drugs. This paper was the first report for the antiviral activity of T. fragilis
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