Sámano-Salazar C, Alemón-Medina R, Chávez-Pacheco JL, Dávila-Borja VM

Language: Spanish
References: 14
Page: 3-8
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ABSTRACT

Background: In our pharmacology laboratory we have used sucralose (Splenda^®) to mask the unpleasant flavour of metformin extemporaneous formulations. However, there have been reports regarding toxicity of sweeteners as Splenda^® in humans.
Objetive: To determine the ability of sucralose to induce cell death in a model of in vitro cultured human cells at the same concentration used in our formula.
Methods: Cells were cultured in 96-well ELISA plates, then incubated with sucralose, stevia or metformin. Cytotoxicity was determined as concentrathe absorbance of formazan using an ELISA reader. Comparison was performed by ANOVA (p ≤ 0.05).
Results: Neither sucralose or stevia induced cell death when combined with metformin.
Conclusion: Both sucralose and stevia howed to be safe for preparation of paediatric extemporaneous formulations.

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