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2015, Number 03

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MediSan 2015; 19 (03)

Proposal of a cytological technique for fast staining

Escalona VR, Ochoa RM
Full text How to cite this article

Language: Spanish
References: 7
Page: 440-443
PDF size: 126.88 Kb.


Key words:

staining methods, cytological techniques, pathology laboratories.

ABSTRACT

In this work a new staining method of Romanowsky type is presented, for the cellular samples obtained through aspirative puncture with needle and the organic liquids, consisting in a modification of the staining technique of May Grünwald-Giemsa, keeping in mind the scarcity of materials and necessary reagents for the preparation of the original procedure and of Diff-Quick® technique in the Pathology laboratories from Santiago de Cuba province. After proving with different dissolutions, by means of the assay and error method, a proportion was obtained which was appropriate for the proposed aims.


REFERENCES

  1. Wu M, Idrees M, Zhang Z, Genden E, Burstein DE. Papanicolaou stain may not be necessary in majority of head and neck fine-needle aspirations: Evidence from a correlation study between Diff-Quik-based onsite diagnosis and final diagnosis in 287 head and neck fine-needle aspirations. Diagn Cytopathol. 2010; 38(11): 846-53.

  2. Gonçalves AJ, Menezes MB, Kavabata NK, Bertelli Antonio AT, Souza Ricardo AS, Joelsons D. Punção aspirativa nos tumores das glândulas salivares: especificidade e sensibilidade. Rev Assoc Med Bras. 2007 [citado 9 Apr 2012]; 53(3): 267-71.

  3. Palomeque Castro B, Ramos Pastor A, Varona Esquivel G. Tinción de Giemsa para histología. Dos protocolos de actuación. Actas del IX Congreso Virtual Hispanoamericano de Anatomía Patológica y II Congreso de Preparaciones Virtuales. 1 May-31 Jun 2007; Ciudad Real. Madrid: CGCOM; 2007 [citado 14 Jun 2012].

  4. Figueredo Thiel S. Diagnóstico citohistoquímico sistemático en biopsias de médula ósea y citologías hematológicas en el Paraguay. An Fac Cienc Méd (Asunción). 2005 [citado 15 Jul 2012]; 38(1-2).

  5. Kocjan G. Fine needle aspiration cytology: diagnostic principles and dilemmas. New York: Springer; 2006.

  6. Parrilla Delgado ME, López Soto MV, Valls Pérez O. Generalidades. En: Atlas de ecocitopatología diagnóstica en las lesiones abdominales. La Habana: Editorial Ciencias Médicas; 2006.

  7. Escalona Veloz R. Punción aspirativa con aguja fina para el diagnóstico de tumores en anatomía patológica. MEDISAN. 2012 [citado 1 Jul 2012]; 16(2).




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C?MO CITAR (Vancouver)

MediSan. 2015;19