2015, Number 3
Growth and survival of total and pathogenic Vibrio parahaemolyticus in American oyster (Crassostrea virginica) under cold storage
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ABSTRACTObjective. To quantify Vibrio parahaemolyticus densities in American oyster (Crassostrea virginica) under cold storage. Materials and methods. 320 oysters were stored at 7 °C for nine days and total and pathogenic densities were determined by the NMP-PCR methodology. Results. V. parahaemolyticus tlh+ densities were observed on 0, 3, and 6 days of storage at 1.134, 2.764 and 0.785 log10NMP/g, respectively, and pathogenic density trh+ on 0 and 3 days at 0.477 and 0.519 log10NMP/g, respectively; the pathogenic densities tdh+ (0.519 log10NMP/g), tdh+/trh+ (0.519 log10NMP/g), and tdh+/orf8+ (-0.444 log10NMP/g) were detected on day 3 of storage. Conclusion. The results suggest that V. parahaemolyticus growth and pathogenic genes occurrence at 7 °C involve changes in the genetic expression as a cold shock response, favoring V. parahaemolyticus survival and virulence, representing a health risk.
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