2001, Number 2
Vet Mex 2001; 32 (2)
Comparison of quantitative buffy coat analysis, thick blood smear and direct observation for the diagnosis of Dirofilaria immitis infection in dogs from three geographical areas of Mexico
Bautista GCR, Arroyo RM, Velasco CO, Canto OL
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ABSTRACTThe objective of this study was to compare three diagnostic tests: the quantitative buffy coat (QBC) analysis, thick blood smear (TBS) and direct observation (DO) to detect canine Dirofilaria immitis infection. Ninety four blood samples were taken from stray dogs from three states of Mexico (48 from Mexico City, 31 from the State of Puebla, and 15 from the State of Tabasco) and were analyzed by QBC, TBS and DO. Additionally, samples from the State of Puebla were also examined by IFAT and ELISA. All samples from Mexico City were negative to D. immitis by QBC, TBS and DO. Twelve samples (38.7%) from the State of Puebla were positive by QBC, 10 (32.3%) by TBS and two (6.4%) by DO. From the samples from the State of Tabasco, 11 (73.3%) were positive by QBC, and ELISA for the heartworm antigen, 10 (66.6%) by IFAT, nine (60%) by TBS, and five (33.3%) by DO. Prevalence rates obtained by QBC were 0%, 38.7%, and 73.3% for Mexico City, Puebla and Tabasco, respectively. In general, the sensitivity obtained was 100%, 82.6% and 30.4% for QBC, TBS, and DO, respectively, while the specificity was 100%, for the three tests. It is concluded that the QBC proved to be a relatively simple method for detecting D. immitis microfilariae in dogs’ blood which could be also useful for the diagnosis of dirofilariosis in human beings.