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2014, Number 4

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Rev Cubana Plant Med 2014; 19 (4)

Evaluation of the mutagenic effects of Passiflora edulis Sims (maracujá) tincture on the Allium cepa test system

Costalonga SA, Pimentel BMC

Language: English
References: 14
Page: 329-337
PDF size: 253.59 Kb.


ABSTRACT

Introduction: ever since prehistoric times, plants have been used by the population to treat all sorts of diseases. One of the most popular medicinal species in Brazil and other Latin American countries is Passiflora edulis Sims, of the family Passifloraceae and known as maracujá, which is widely used to treat nervous disorders. However, in order to validate its safety it is necessary to determine whether the plant has the capacity to change the cell cycle and cause toxic effects on the genetic material.
Objective: evaluate the toxic, aneugenic and clastogenic effect of Passiflora edulis Sims tincture on the initial development and mitotic index of meristematic onion root cells using the Allium cepa test system.
Methods: Allium cepa seeds were subjected to continuous and discontinuous treatments (acute - 20 h and chronic - 72 h) with distilled water (witness), 20 % alcohol and three concentrations of Passiflora edulis tincture (6, 12 and 24 drops).
Results: the mitotic index showed a significant reduction at concentrations of 6 drops (continuous treatment), 12 and 24 drops (acute discontinuous treatment - 20 h), and all concentrations in the chronic discontinuous treatment - 72 h. The aneugenic effect occurred at concentrations of 24 drops in both discontinuous treatments, whereas a clastogenic effect was observed at concentrations of 12 and 24 drops in the two discontinuous treatments.
Conclusions: the tincture showed great mutagenic potential, and was responsible for inducing damage to the cell cycle. Validation of its use in human beings requires further testing.


REFERENCES

  1. Ferro D. Fitoterapia: conceitos clínicos. São Paulo: Atheneu; 2006. 502 p.

  2. Lorenzi H, Matos FJA. Plantas medicinais no Brasil: nativas e exóticas cultivadas. 2nd Ed. São Paulo: Instituto Plantarum; 2008. 560 p.

  3. Gurib-Fakim A. Traditional Roles and Future Prospects for Medicinal Plants in Health Care. Asian Biotechnology and Development Review. 2011;13(3):77-83. [Access in 19/09/2012]. Available from: http://www.cephyr-recherche.com/wpcontent/ uploads/2012/11/ABDRNOV11AGF.pdf

  4. Bezerra AMF, Bezerr KKS, Sousa LCFS, Sousa JS, Borg MGB. Plantas medicinais utilizadas pela comunidade de mimoso no município de Paulista, Paraíba – Brasil. Revista Verde. 2012;7(5):06-11. [Access in 20/03/2013]. Available from: http://www.gvaa.org.br/revista/index.php/RVADS/article/viewFile/1856/pdf_539

  5. Bruning MCR, Mosegui GBG, Vianna CMM. A utilização da fitoterapia e de plantas medicinais em unidades básicas de saúde nos municípios de Cascavel e Foz do Iguaçu – Paraná: a visão dos profissionais de saúde. Ciência & Saúde Coletiva. 2012;17(10):2675-2685. [Access in 20/03/2013]. Available from: http://www.scielosp.org/pdf/csc/v17n10/17.pdf

  6. Martins ER, Castro DM, Castellani DC, Dias JE. Plantas medicinais. Viçosa: UFV; 2003. 220 p.

  7. Simões CMO, Schenkel EP, Gosmann G, Mello JCP, Mentz LA, Petrovick PR. Farmacognosia: da planta ao medicamento. 6th ed. Florianópolis: UFSC; 2010. 1104 p.

  8. Braga A, Medeiros TP, Araújo BV. Investigação da atividade antihiperglicemiante da farinha da casca de Passiflora edulis Sims, Passifloraceae, em ratos diabéticos induzidos por aloxano. Braz. J. Pharmacogn. 2010;20(2):186-191. [Access in 28/07/2011]. Available from: http://www.scielo.br/pdf/rbfar/v20n2/a09v20n2.pdf

  9. Braz Filho R. Contribuição da fitoquímica para o desenvolvimento de um país emergente. Quim Nova. 2010;33(1):229-239.

  10. Coelho MFB, Maia SS, Oliveira AK, Diógenes FEP. Atividade alelopática de extrato de sementes de juazeiro. Hortic. Bras. 2011;29(1):108-111. [Access in 15/11/2012]. Available from: http://www.scielo.br/pdf/hb/v29n1/18.pdf

  11. Martins CM, Vasconcellos MAS, Rossetto CAV, Carvalho MG. Phytochemical screening of the arils of yellow passion fruits seeds and influence on the seed germination. Ciência Rural. 2010;40(9):1934-1940. [Access in 19/09/2012]. Available from: http://www.scielo.br/pdf/cr/v40n9/a720cr2740.pdf

  12. Ribeiro LR, Salvadori DMF, Marques EK. Mutagênese Ambiental. Canoas: ULBRA, 2003. 356 p.

  13. Marin-Morales MA, Leme DM. Allium cepa test in environmental monitoring: A review on its application. Mutation Research. 2009;682:71-81. [Access in 20/10/2011]. Available from: www.elsevier.com/locate/reviewsmr

  14. Fiskejo G. The Allium test as a standard in environmental monitoring. Hereditas (Lund). 1985;(102):99-112.




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