medigraphic.com
ISSN 1028-4796 (Print)

2014, Number 4

<< Back Next >>

Rev Cubana Plant Med 2014; 19 (4)

Antinociceptive and antibacterial activity of total alkaloids from two species of the Solanaceae family

Soto VMR

Language: Spanish
References: 29
Page: 361-373
PDF size: 231.54 Kb.


ABSTRACT

Introduction: Solanum multifidum Lam. and Lycianthes lycioides (L.) Hassl. are two species typical of coastal hills of Peru. Both belong to the Solanaceae family, and are renowned for their high content of alkaloids with a variety of therapeutic activities. That was the reason why the present study was conducted.
Objective: evaluate the antinociceptive and antibacterial activity of total alkaloids from leaves of Solanum multifidum Lam. and Lycianthes lycioides (L.) Hassl.
Methods: total alkaloids were extracted from dry leaves of the study species following the method described by Peña. Antinociceptive activity was evaluated in vivo using the writhing test, whereas in vitro antibacterial activity was evaluated with the agar diffusion technique with impregnated discs. Minimum inhibitory concentration was determined by the agar dilution method.
Results: in both species total alkaloids showed antinociceptive activity at doses of 2.5 mg/kg, 5 mg/kg y 10 mg/kg, with the greatest inhibition percentage at 10 mg/kg and statistically significant differences (p ‹ 0.05). The alkaloids also inhibited the growth of Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25992) and Pseudomona aeruginosa (ATCC 27853) at concentrations of 2 mg/mL and 4 mg/mL, exhibiting greater bioactivity against Staphlylococus aureus. In general terms, total alkaloids Lycianthes lycioides (L.) Hassl. showed the greatest antinociceptive and antibacterial activity.
Conclusions: it was demonstrated that total alkaloids from both species have antinociceptive and antibacterial activity. The present paper is the first report about bioactivity of these plant species, an attractive option for the development of new drugs.


REFERENCES

  1. Goldberg D, McGee S. Pain as a global public health priority. BMC Public Health. 2011;11(770):1-5.

  2. Edwin E, Sheeja E, Toppo E, Tiwari V, Dutt K. Efecto antimicrobiano, antiulceroso y antidiarreico de las hojas de buganvilla ( Bougainvillea glabra Choisy). Ars. Pharm. 2007;48(2):135-144.

  3. Ruiz J, Villamil L. Enfermedades emergentes y barrera de especies: Riesgo del Herpesvirus equino 9. Rev. Salud pública. 2008;10(5):840-847.

  4. Newman D, Cragg G. Natural Products as Sources of New Drugs over the Last 25 Years. J. Nat. Prod. 2007;70(3):461-477.

  5. Jerzykiewicz J. Alkaloids of Solanaceae (nightshade plants). Postepy Biochem. 2007;53(3):280-286.

  6. Bennett J. Revision of Solanum section regmandra (solanaceae). Edinburgh Journal of Botany. 2008;65(1):69-112.

  7. Mitchell G, Gattuso M, Grondin G, Marsault E, Bouarab K, Malouin F. Tomatidine Inhibits Replication of Staphylococcus aureus Small-Colony Variants in Cystic Fibrosis Airway Epithelial Cells. Antimicrob Agents Chemother. 2011;55(5):1937-1945.

  8. Pandurangan A, Khosa R, Hemalatha S. Antinociceptive activity of steroid alkaloids isolated from Solanum trilobatum Linn. J Asian Nat Prod Res. 2010;12(8):691-695

  9. Parma K, Bindu S, Nidhi B. Biological activity of alkaloids from Solanum dulcamara L. Nat. Prod. Research. 2009;23(8):719-723.

  10. Alarcón L, Velazco J, Usubillaga A. Determinación de la actividad antibacteriana de los alcaloides presentes en los frutos verdes de Solanum hypomalocophyllum Bitter. Rev. Latinoamer. Quím. 2006;34(1-3):13-21

  11. Ripperger H, Porzel A. 2α-Hydroxysoladulcidine from Lycianthes biflora. Phytochemistry. 1992;31(2):725-726.

  12. Peña W. Evaluación del contenido de glicoalcaloides en el pelado, cocción y fritura de variedades de papa nativa. [Tesis], Ecuador: Escuela Politécnica Nacional del Ecuador; 2010. [citado 9 de marzo 2013]. Disponible en: http://eelalnx01.epn.edu.ec/bitstream/15000/2636/1/CD-3319.pdf

  13. Asociación Médica Mundial. Declaración de la AMM sobre el uso de animales en la investigación biomédica. Sudáfrica: Asociación Médica Mundial. 2006. [citado 12 de marzo 2013]. Disponible en: http://www.wma.net/es/30publications/10policies/a18/

  14. Salazar A, Santa María J, Zimic C, Salinas I, Sánchez L, Arrambide J, et al. Antinocicepción opioide del extracto metanólico del Chuchuhuasi (Maytenus krukovii) en un modelo de dolor visceral en ratón. Rev Horizonte Médico. 2006;6(2):135-140.

  15. Gracia C, Correa E, Rojas N. Estudio fitoquímico preliminar y evaluación antimicrobiana de algunas plantas superiores colombianas. Rev. Colom. Ciencias quim. Farm. 1995;23(1):42-48.

  16. Meurer B, McBeth D, Hallihan B, Delph S. Antimicrobial activity in medicinal plants of the Escrophulariaceae and Acanthaceae. Int. J. Pharmacog. 1996;34(4):243-248.

  17. Martínez M, González A, Cazares L, Moreno M, García A. Antimicrobial Activity of Byrsonim acrassifolia (L) H.B.K. J. Ethnopharmacol. 1999;66(1):79-82.

  18. Corzo D. Evaluation of antimicrobial activity of ethanol extract of Cestrum buxifolium Kunth. Rev Mex Cienc Farm. 2012;43(3):81-86.

  19. Marrassini C, Gozalczany S, Ferraro G. Actividad analgésica de dos especies de Urtica con usos etnomédicos en la República Argentina. Dominguezia. 2010;26(1):21-29.

  20. Marrassini C, Acevedo C, Miño J, Ferraro G, Gorzalczany S. Evaluation of antinociceptive, antinflammatory activities and phytochemical analysis of aerial parts of Urticaurens L. Phytother Res. 2010;24(12):1807-12.

  21. Nguemfo E, Dimo T, Azebaze A, Asongalem E, Alaoui K, Dongmo A, et al. Antiinflammatory and antinociceptive activities of the stem bark extracts from Allanblackia monticola STANER L.C. (Guttiferae). J. Ethnopharmacol. 2007;114(3):417-424.

  22. Bezerra M, Lima V, Girao V, Teixeira R, Graca J. Antinociceptive activity of sildenafil and adrenergic agents in the writhing test in mice. Pharmacol. Rep. 2008;60(3):339-344.

  23. Martín M. Determinación de glicoalcaloides: α-solanina y α-chaconina en patata mediante cromatografía de líquidos de ultra presión acoplada a espectrometría de masas de triple cuadrupolo. [Tesis de Maestría], España: Universidad de Almería. 2011. [citado 24 de mayo 2012]. Disponible en: http://repositorio.ual.es/jspui/bitstream/10835/491/1/DETERM~1.PDF

  24. Olortegui J, Brañez M. Evaluación de la actividad citotóxica y antimicrobiana de glicoalcaloides esteroidales de las hojas de Solanum albidum Dunal y Solanum oblongifolium Dunal. [Tesis], Perú. Universidad Nacional Mayor de San Marcos. 2009. [citado 10 de marzo 2013].Disponible en: http://www.cybertesis.edu.pe/sisbib/2009/branez_em/pdf/branez_em.pdf

  25. Niño J, Correa Y, Mosquera O. Biological activities of steroidal alkaloids isolated from Solanum leucocarpum. Pharm. Bio. 2009;47(3):255-259.

  26. Chakraborty A, Branert, H. Antibacterial steroid alkaloids from the stem bark of Hallarrhena pubescens. J Ethnopharmacol. 1999;68(1-3):339-344.

  27. Kim Y, Che Q, Gunatilaka A, Kingston D. Bioactive steroidal alkaloids from Solanum umbelliferum. J Nat Prod. 1996;59(3):283-285.

  28. Silva N, Ruíz S, López M, Cira L, Gortárez P. Componentes bioactivos de residuos de papa: un recurso para la desinfección de las aguas. Ide@s CONCYTEG. 2011;6(71):561-570.

  29. Keukens E, Vrije T, Van den Boom C, De Waard P, Plasman H, Thiel F, et al. Molecular bases of glycoalkaloid induced membrane disruption. Biochim Biophys Acta. 1995;1240(2):216-228.




2020     |     www.medigraphic.com