Rinflerch AR, Flores V, Argibay PF, Galimberti RL

Language: Spanish
References: 9
Page: 108-110
PDF size: 134.25 Kb.

ABSTRACT

Onychomycosis affects 5-20% of the world population. It could be caused by three types of fungi: dermatophytes, yeasts, and molds; being the dermatophytes, the most commonly found. The aim of this study was to evaluate the efficacy and sensitivity of real time pcr (rt pcr) to identify specie and gender Trichophyton rubrum and T. interdigitale, and the incidence of dermatophytes as causing agent of onychomycosis in a sample of argentinian population.
A total of 60 clinical samples were examined by both microscopic and rt pcr (using dna extracted directly from clinical samples). In 58 (95%) of the samples, fungal dna was detected by rt pcr, while only 41 (68%) were positive by microscopy. Out of 58 rt pcr positive clinical samples, 53 (91%) were identified as T. rubrum and 5 (9%) as T. interdigitale.
The rt pcr approach is more sensitive to detect dermatophytes in a clinical sample than the microscopic method, and faster than conventional culture. However, false positive results are a concern, since this method cannot evaluate the viability of the fungi. Then, rt pcr approach may be useful for non-treated patients.

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