2015, Number 3
Rev Cubana Plant Med 2015; 20 (3)
Antioxidant activity, total phenolic content and cytotoxicity of polar extracts from colombian antidiabetic plants
Rivas MKE, Muñoz DL, Pino BCN, Balcázar MN
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ABSTRACTIntroduction: the traditional use of plants as antidiabetic herbal medicine is common practice in the Colombian Pacific, A. altilis, B. picta, S. malaccensis, P. microphylla y P. quadrangularis are five species that are widely recognized as natural antidiabetic regionally, without the existence experimental evidence to validate that assessment.
Objective: evaluate the total content of phenolic compounds, potential antioxidant and cytotoxic activity of polar extracts prepared from five plant species used as natural antidiabetic Colombian traditional medicine.
Methods: extracts of leaves (leaves and stems for P. microphylla) were obtained by maceration in 96% ethanol or distilled H2O as appropriate. The antioxidant activity was evaluated by spectrophotometric assays DPPH 2, 2-diphenyl-1-picrilhidracil and ABTS 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), while the total content of phenolic molecules is determined by the Folin-Ciocalteu method. Furthermore, the cytotoxic activity was tested in the Hep-G2 cell line (human hepatocarcinoma) and C2C12 (mouse Miocarcinoma).
Results: the ethanol extracts of A. altilis, S. malaccensis and B. picta, showed the best antioxidant behavior in both methods, with properties comparable to vitamins E and C. Likewise, as to the toxic properties of the extracts A. altilis reported the highest percentage of mortality in C2C12 cells with an LC50 value of 24,9 ± 0,67 mg/mL, comparable to the cytotoxicity exerted by amphotericin B, while in HEP-G2 cells the major toxic effect was governed by P. quadrangularis with LC50 of 36,4 ± 5,79mg/mL.
Conclusions: the potential antioxidant evidenced by the ethanol and aqueous extracts of A. altilis, S. malaccensis, B. picta, P. quadrangularis and P. microphylla in 62.39% correlated with the total content of phenolic molecules. All extracts showed fifty cytotoxic activitiesat concentrations greater than their antioxidant activityrange.