2015, Number 3
Rev Cubana Plant Med 2015; 20 (3)
Peña NBR, Duménigo GA, Martínez HI
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ABSTRACTIntroduction: Punica granatum L. is known in Cuba as grenade. In traditional medicine, among other properties, antiflu action is attributed to this plant. In previous studies with the hydroalcoholic lyophilized extract, prepared with the fruit of this specie, several chemical compounds were reported, specially the presence of flavonoids. The variety of secondary metabolites detected for this lyophilized extract, added to a flavonoid fraction confers a high possibility of anti-influenza activity, as has been demonstrated in previous studies.
Objective: to evaluate the virucidal activity of the hydroalcoholic lyophilized extract of P. granatum against Influenza A/Mississippi/1/85 (H3N2), with different schemes of treatments.
Methods: the different schemes of treatments included 27 variants of the variables such as contact time of 60, 30 and 15 minutes; extract concentration of 1000, 200 and 125 µg/mL; an incubation temperature of the mix extract-virus of 4, 25 and 37°C; and the corresponding replicas. The presence or not of hemaglutinin of influenza virus in each one of the treated samples, was measured by titration of Hemaglutination Inhibition (HAI) and the Embryo Infection Doses (EID50)
Results: the minimal effective concentration to show in the hydroalcoholic lyophilized extract the direct virucidal action in vitro was 125 µg/mL, in 15 minutes of contact. This virucidal action was always present in the different assessed temperature and time exposures.
Conclusions: the treatment of 125 µg/mL, regardless of time and temperature of treatment, was effective enough to reduce the presence of hemaglutinin of the influenza virus.