2003, Number 4
Acta Ortop Mex 2003; 17 (4)
Osteoinductive effect of rat recombinant leptin in an experimental model with tibial and fibula fractures: Pilot study
Franco-Boruland RE, Vergara FHJ
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ABSTRACTThere is solid evidence for a relation between leptin and the regulation of bone metabolism. Here we have explored in an experimental model ad hoc the possibility of an effect of rat recombinant leptin (rr-l) on fracture healing in the rat. Under anesthesia, the right tibia and fibula of eight Long Evans rats of 450-500 g body weight (bw) were surgically fractured. Tibial fractures were stabilized with a centromedullary Kirschner wire (0.035). Four randomly chosen rats received rr-l locally to the fractured member (L-rats): 100 µg/kg bw immediately after surgery and 20 µg/kg bw/day for the following 15 days; four control (C) rats received only the vehicle. Contrary to the C-rats, the 2 L-rats that survived the treatment with rr-l were walking normally 15 days after being fractured; at the incision site their skin was healed. All rats were sacrificed 22 days after surgery. The fractured bones were dissected out and fixed in 2.5% glutaraldehyde. The tibiae and fibulae of the L-rats were consolidated, as opposed to those of the C-rats. All rats suffered an infectious process in the fractured member, although the L-rats showed a notably preserved articular surface of the tibia.