Estrada GG, Reyes TR, Maida CR, Beltrán MJ, Vadillo OF

Language: Spanish
References: 15
Page: 198-204
PDF size: 91.71 Kb.

ABSTRACT

Objective: To identify whether soluble products from choriodecidual blood cells stimulated with group B streptococci (GBS) induce connective tissue degradation in human amniochorion.
Material and methods: Blood samples from choriodecidual compartment were collected by direct aspiration from placental cotyledons draining blood and represent local circulating cells. Samples were divided into two aliquots: one was stimulated with GBS (1X10⁶ CFU/mL) and the other was kept free of bacteria as negative control. After overnight incubation, plasmas were separated. Chorioamnion explants were stimulated with 10% plasma for 12h at 37°C in 5% CO₂. MMP-9 proteolytic activity was measured in the supernatants by gelatin-zymography and IL-1β and TNF-α were quantified by ELISA. Distribution of the collagenous fibrils in explants was examined by electron microscopy. Statistical analysis: three independent experiments on duplicate were carried out and the statistical significance of experimental differences between groups was assessed with ANOVA test.
Results: MMP-9, IL-1β and TNF-α production was significantly higher in supernatants from explants co-cultured with choriodecidual plasma from blood previously infected with GBS, compared with control plasma. Accompanying extensive changes of connective tissue arrangement confirm induction of extracellular matrix degradation.
Conclusions: Choriodecidual plasma from blood stimulated with GBS is enriched with biochemical signals that enhance the MMP-9, IL-1α and TNF-β production by amniochorion. These findings suggest that local circulating cells are capable to act in response to GBS choriodecidual infection through extracellular matrix degradation and the consequent rupture of membranes.

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