Rodríguez MCE, Pulido SNJ

Language: Spanish
References: 21
Page: 375-388
PDF size: 158.09 Kb.

ABSTRACT

Introduction: the Morus alba (L.) and Gliricidia sepium was used as an alternative in vitro control of the adult tick Rhipicephalus (Boophilus) microplus.
Objective: evaluate the efficacy of the extract of Morus alba and Gliricidia sepium in vitro control adult tick Rhipicephalus (Boophilus) microplus and oviposition.
Methods: the leaves of each plant was used dry, each plant extract and hydroalcoholic was obtained by maceration techniques. Assays were in vitro by immersion technique adult, pure extract and 1:2 dilution was used. Adult ticks that were exposed to extracts from each plant were used. at 24, 48, 72 and 96 h of exposure, performed the reading mortality, it was taken as effective minimum mortality of 60%, to assess inhibition of oviposition ticks were placed in the dorsal position in number of 10 on a petri dish, weighing the eggs past day 15 was performed days of this process, the capacity of each extract was assessed inhibit oviposition. Qualitative tests were performed to determine the presence of secondary metabolites.
Results: pure mulberry extract showed a mortality of 73.3% and 1:2 dilution with a mortality of 40% and matarratón extract showed 53.33% mortality in adult tick; for inhibition of oviposition pure mulberry extract showed 73.01% inhibition for 1:2 dilution and showed 26.21% inhibition and the pure matarratón extract showed 58 % inhibition.
Conclusions: the extract showed the best mortality rate of Rhipicephalus (Boophilus) microplus and inhibition in oviposition was obtained with the Morus alba plant and presence of alkaloids and coumarins.

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