Rincón MCA, Castaño OJC, Gómez BM, Rezek J, Vanĕk, T

Language: Spanish
References: 13
Page: 409-418
PDF size: 236.18 Kb.

ABSTRACT

Introduction: Acmella ciliata (Kunth) Cass is a weed renowned as a famous folk remedy for toothache and throat. The chemical substances which are attributed their biological activity are aliphatic alkamides.
Objetivo: analyze the chemical composition and biological activity of a fraction rich in alkamides from the apolar extract of Acmella ciliata (Kunth) Cass flowers.
Methods: the qualitative analysis of metabolites was carried out using two-Dimensional gas chromatography coupled to time-of-flight mass spectrometry (CGxCG/TOFMS).The biological activity was evaluated by the minimum inhibitory concentration assay (MIC) using the resazurin microplate method.
Results: the chloroformic fraction of the etheric extract from A. ciliata flowers presents a variety of nitrogen containing compounds including predominantly aliphatic alkamides. N-Isobutyl-2 (E), 6 (Z), 8 (E)-decatrienamide (spilanthol) was the most abundant amide within the group of 5 alkamides found. Regarding its biological activity, the chloroformic fraction exhibited antimicrobial activity against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans.
Conclusions: chloroform fraction of A. ciliata shows great potential because of its chemical and pharmacological properties. Although all tested bacterias and fungi were inhibited by the activity of alkamides separated, C. Albicans was the most susceptible microorganism to the antimicrobial activity of the fraction, demonstrating a MIC of 312.5 µg/mL.

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