medigraphic.com
ISSN 1028-4796 (Print)

2016, Number 1

Next >>

Rev Cubana Plant Med 2016; 21 (1)

Modulatory activity of ethanolic extracts of the leaves of Clusia nemorosa G. Mey (Clusiaceae) on antimicrobial drugs

Honorio OA, Oliveira AA, Cortez SVL, Pessoa SMA, Melo CHD, Figueiredos SMA

Language: Portugu?s
References: 19
Page: 1-8
PDF size: 126.04 Kb.


ABSTRACT

Introduction: the fast development of drug resistance and the slowdown of the development of new active drugs, drew attention to the treatment with the drug combination.
Objective: to analyze the antimicrobial activity of the ethanol extract of Cluisa nemorosa (Clusiaceae) against multiresistant strains and standards as well as the modulating action with aminoglycoside amikacin, gentamicin and neomycin.
Methods: the plant material (leaves) was collected in Araripe, crushed and put into ethanol 96 % as solvent. Distillation of the solvent was then performed in a rotary evaporator to produce the gross ethanol extrato. A microdilution assay was conducted to verify antibacterial activity and the possible interactions of aminoglycosides associated with the study samples. A sub-inhibitory concentration of 128 µg/mL (Minimum Inhibitory concentration/8) was used.
Results: the activity of the extract against the strains isolated patterns had an Minimum Inhibitory concentration › 1024 mg/mL, the action of antibiotics was modulated synergistically by the extract against multidrug-resistant Gram-positive bacteria Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa.
Conclusions: the synergism of the ethanol extract was verified by microdilution method. Therefore, it is suggested that the extract of Clusia nemorosa be used as a source of natural products for antimicrobial therapy and to combat bacterial multidrug resistance.


REFERENCES

  1. Freitas AG, Farias ET, Lima MCA, Sousa IA, Ximenes, EA. Atividade antiestafilocócica do Plantago major L. In: Revista Brasileira de Farmacognosia. 2002;12:64-5.

  2. Keith CT, Borisy AA, Stockwell BR. Multicomponent therapeutics for networked systems. Nat Rev Drug Discovery. 2005;4:71-8.

  3. Nascimento GGF, Lcatelli J, Freitas PC, Silva GLS. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Rio de Janeiro: Braz. J. Microbiol. 2000;31(4):247-56.

  4. Phillipson JD, Anderson LA. Ethnopharmacology and western medicine. Journal Ethnopharmacology. 1989;25:61-72.

  5. Zuanazzi JAS, Montanha JA. Flavonóides. Farmacognosia: da planta ao medicamento. 5. ed. rev. ampl. Florianópolis: Ed. da UFSC; Porto Alegre: Ed. da UFRGS; 2004. p. 577-614.

  6. Stevens PF. Cluisaceae-Guttiferae. In: K. Kubitzki, (ed.). The familes and genera of vacular plants. Berlin: Springer. 2007;9:48-66.

  7. Suffredini IB, Paciencia MLB, Nepomuceno DC, Younes RN, Varella AD. Antibacterial and cytotoxic activity of Brazilian plant extracts -Clusiaceae. Rio de Janeiro: Mem Inst Oswaldo Cruz. 2006;101(3):287-90.

  8. Javadpour MM, Juban MM, Lo WC, Bishop SM, Alberty JB, Cowell SM, et al. De novo antimicrobial peptides with low mammalian cell toxicity. J Med Chem. 1996;39:3107-13.

  9. Salvat A, Antonnacci L, Fortunato RH, Suarez EY. Screenng of some plants from northern argentina for their antimicrobial activty. Letters in Applied Microbiology. 2001;32(5):293-7.

  10. CLSI. National committee for clinical laboratory standards. Performance standards of antimicrobial disk susceptibility test, 8th ed. Atlanta, USA: CLSI; 2003. p. 2-8.

  11. Hadacek F, Greger H. Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochemical Analysis. 2000;11:137-47.

  12. Houghton PJ, Howes MJ, LEE CC, Steventon G. Uses and abuses of in vitro tests in ethnopharmacology: Visualising an elephant. J Ethnopharmacol. 2007;110:391–400.

  13. Ribeiro PR, Ferraz CG, Guedes ML, Martins D, Cruz FG. A new biphenyl and antimicrobial activity of extracts and compounds from Clusia burlemarxii. Fitoterapia. 2011;82:1237-40.

  14. Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Prod. 1996;59:205-15.

  15. Dixon RA, Dey PM, Lamb CJ. Phytoalexins: enzymology and molecular biology. Advances in Enzymology and Related Areas of Molecular Biology. 1983;55:1–69.

  16. Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, et al. Comparative study on the antibacterial Activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology. 1996;50:27–34.

  17. Carmo RM, Franceschilene EV. Polinização e biologia floral de Clusia arrudae Planchon & Triana (Clusiaceae) na Serra da Calçada, município de Brumadinho, MG. Revista Brasileira de Botânica. 2002;25:505-13.

  18. Lokvan J, Braddock JF, Reichardt RB, Clausen TP. Two polyisoprenylated benzophenones from the trunk latex of Clusia grandiflora (Clusiaceae). Phytochemistry. 2000;55:29-34.

  19. Pinheiro L, Cortez DAG, Vidotti GJ, Young MCM, Ferreira AG. Estudo fitoquímico e avaliação da atividade moluscicida da Kielmeyera variabilis Mart (Clusiaceae). Química Nova. 2003;26:157-60.




2020     |     www.medigraphic.com