Bermúdez DL, Cuéllar CA, Licham MA, Huamán SJ

Language: Spanish
References: 13
Page: 31-41
PDF size: 412.85 Kb.

ABSTRACT

Background: the traditional Peruvian medicine has been used since ancient times to Gentianaceae species for Diabetes Mellitus treatment. These include the Gentianella bicolor (Wedd.) Fabris ex JS Pringle (G. bicolor), known as "corpus huay”, “campanilla", "shashacuma", “campanilla morada ". This plant, botanically described as biennial herb, perennial, up to 60 cm tall, woody root, has been reported in the region of Cajamarca, Peru and in Bolivia between 3000 and 4500 metres above sea level.
Objective: to evaluate the hypoglycemic effect of the G. bicolor aqueous extract in streptozotocin (STZ)-induced diabetic rats.
Methods: in this study Twenty-eight Sprague Downley (SD) (9 weeks and weighing 200-210 g) were tested. Sprague Downley were divided into 04 experimental groups; 01 control group consisted of 07 healthy Sprague Downley and other groups consisted were STZ-induced diabetic Sprague Downley by intraperitoneal injection of Streptozotocin (60 mg/Kg B.W). A group was taken as untreated STZ-diabetic positive control SD, another STZ-induced diabetic group received glibenclamide (10 mg/kg B.W.), and the other group received the aqueous extract of G. bicolor (500 mg/Kg B.W.) daily for 21 days.
Results: from day 14 of de experimentation, a significant decrease in blood glucose was observed in the experimental group treated with G. bicolor aqueous extract, showing a decrease about 40 %, 06 hours after the extract administered. The histopathological examination showed that the pancreas of STZ-diabetic SD treated with the aqueous extract of G. bicolor had a pancreatic homogeneous structure with the presence of islets virtually normal size.
Conclusion: G. bicolor aqueous extract have a chronic hypoglycemic effect and a possible regenerative effect of the pancreatic islets.

REFERENCES

1. American Diabetes Association. Classification and Diagnosis of Diabetes. Diabetes Care. 2015;38(1):S8-16.

2. Sacks D, Arnold M, Bakris G. Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus. Diabetes Care. 2011;34:61-99.

3. Ke-wei Wang. Assessment of the Magnitude of Contextual and Individual Demographic Effects on Diabetes Mellitus and Glucose Intolerance in Rural Southwest China: A Multilevel Analysis. PLoS ONE. 2013;8(7):6855-65.

4. Bussmann R, Paniagua-Zambrana N, Chamorro M, Moreira N, del Rosario Cuadros Negri M, Olivera J, et al. Peril in the market-classification and dosage of species used as anti-diabetics in Lima, Peru. J Ethnobiol Ethnomed. 2013;9:37.

5. León B, Roque J, Ulloa C. El Libro Rojo de las Plantas Endémicas del Perú. Revista Peruana de Biología. 2006;13(2):339-54.

6. Mostacero León J, Castillo Picón F, Mejía Coico FR, Gamarra Torres A, Charcape Ravelo JM, Ramírez Vargas RA, et al. Plantas Medicinales del Perú. Taxonomía, Ecogeografía, Fenología y Etnobotánica. Perú: Asamblea Nacional de Rectores ed; 2011.

7. Brako L, Zaruchi J. Catalogue of the flowering Plants and Gymnosperm of Peru. United States of America: Missouri Botanical Garden ed; 1993.

8. Salazar Díaz J. Contribución al Estudio Químico y Farmacológico de la Gentianella umbellata (G. Don) Fabris. Tesis presentada por Para optar el Grado Académico de Magister en Química: Pontificia Universidad Católica del Perú; 2011.

9. Becerra Juan. Estudio morfo-histotaxonómico e identificación de los fitoconstituyentes de la especie Gentianella alborosea (Gilg) Fabris “Hercampuri”. Tesis para optar el título de Químico Farmaceútico, Universidad Nacional de Trujillo; Perú 1991.

10. Miranda M, Cuéllar A. Farmacognosia y Productos naturales. Cuba: EIMAV, Ministerio de Educación ed; 2000.

11. Bancroft J, Gamble M. Theory and Practice of Histological Techniques, 5th ed. Edinburgh: Churchill Livingstone; 2001.

12. Tomas G. Estudio fitoquímico y farmacológico de la Gentianella thyrsoidea Hooker fabris. Tesis para optar por el título de Magister en Química. Pontificia Universidad Católica del Perú; 2000.

13. Alimohammadi S, Hobbenaghi R, Javanbakht J, Kheradmand D, Mortezaee R, Tavakoli M, et al. Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ)- induced diabetic in rats: an experimental study with histopathological evaluation. Diagnostic Pathology. 2013;8:137-44.