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2016, Number 1

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Rev Cubana Plant Med 2016; 21 (1)

Study of in vitro antioxidant capacity of Phaseolus vulgaris L. (frijol) by microwave assisted extraction

Puertas MMA, Mosquera-Mosquera N, Rojano B

Language: Spanish
References: 15
Page: 42-50
PDF size: 164.02 Kb.


ABSTRACT

Introduction: Phaseolus vulgaris L. is a representative crop of nutrient and economic importance in Colombia. Additionally, P. vulgaris is considered as a natural source of bioactives compounds, such as polyphenols, which have been associated with valuable effects on health.
Objetives: to evaluate the microwave extraction assisted technique as an alternative methodology to study the antioxidant capacity of eight varieties of P. vulgaris cultivated in Colombia.
Methods: dehydrated and powered seeds of P. vulgaris was subjected to microwave assisted extraction and solid-liquid extraction and. Total phenolis content was determined by Folin-Cicoulteau method and the potential antioxidant was evaluated using diphenyl-picrylhydrazyl radical stable and 2,2-Azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) radical cation assays.
Results: microwave assisted extraction-Household microwave oven technique was more efficient and versatile than SLE method. The extracts obtained microwave assisted extraction-Household microwave oven methodology showed polyphenol content ranged between entre 29,36 and 60,61 g EGA/L, but SLE was over 32,75 and 113,27 g EAG/L.
Conclusions: all extracts showed a considerable antioxidant potential against free radical, and microwave assisted extraction-Household microwave oven method could be used as an alternative method for fast, efficient and effective evaluation of the content of polyphenol in various matrices, with minimal differences comparing to established microwave assisted extraction techniques.


REFERENCES

  1. Arias-Restrepo JH, Rengifo-Martínez T, Jaramillo-Carmona M. Manual Técnico: Buenas Prácticas Agrícolas en la Producción de Fríjol Voluble. Corporación Colombiana de Investigación Agropecuaria-CORPOICA. Bogotá: CTP Print Ltda; 2007.

  2. Geil P, Anderson J. Nutrition and health implications of dry beans: a review. J Am Coll Nutr. 1994;13(6):549-58.

  3. Mishra SB, Rao CV, Ojha SK, Vijayakumar M, Verma A. An analytical review of plants for anti diabetic activity with their phytoconstituent and mechanism of action. Internat J Pharmacol Sci Res. 2010;1(1):29-44.

  4. Díaz AM, Caldas GV, Blair MW. Concentrations of condensed tannins and anthocyanins in common bean seed coats. Food Res Internat. 2010;43 (2):595-601.

  5. Lin L-Z, Harnly JM, Pastor-Corrales MS. The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chem. 2008;107(1):399-410.

  6. Padilla FC, Rincón AM. Contenido de polifenoles y actividad antioxidante de varias semillas y nueces. Arch Latinoam Nutr. 2008;58(1):303-8.

  7. Buldini PL, Ricci L, Sharma JL. Recent applications of sample preparation techniques in food analysis. J Chromatogr A. 2002;975(1):47-70.

  8. Kataoka H. New trends in sample preparation for clinical and pharmaceutical analysis. TrAC Trend Anal Chem. 2003;22(4):232-44.

  9. Smith RM. Before the injection-modern methods of sample preparation for separation techniques. J Chromatogr A. 2003;1000(1-2):3-27.

  10. Bose AK, Banik BK, Lavlinskaia N, Jayaraman M, Manhas MS. More chemistry in microwave. ChemTech. 1997;27(9):18-23.

  11. LeBlanc G. Microwave-accelerated techniques for solid sample extraction. LC-GC 1999;17(1):30-7.

  12. Ganzler K, Salgó A, Valkó K. Microwave extraction. A novel sample preparation method for chromatography. J Chromatogr A. 1986;371(1):299-306.

  13. Stevenson DG, Inglett GE, Chen D, Chena D, Biswasb A, Ellerc FJ, et al. Phenolic content and antioxidant capacity of supercritical carbon dioxide-treated and airclassified oat bran concentrate microwave-irradiated in water or ethanol at varying temperatures. Food Chem. 2008;108(1);23-30.

  14. Pereira MP, Tavano OL. Use of different spices as potential natural antioxidant additives on cooked beans (Phaseolus vulgaris). Increase of DPPH radical scavenging activity and total phenolic content. Plant Foods Hum Nutr. 2014;69(4);337-43.

  15. Sutivisedsak N, Cheng HN, Willett JL, Willett JL, Lesch WC, Tangsrud RR, et al. Microwave-assisted extraction of phenolics from bean ( Phaseolus vulgaris L.). Food Res Internat. 2010;43(2);516–9.




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