Carrión DIR, García BL, Suárez PY, Rodríguez FB, Aja MG

Language: Spanish
References: 13
Page: 618-629
PDF size: 189.21 Kb.

ABSTRACT

Introduction: creatinine is an important control and monitoring marker in various kidney pathologies.
Objectives: to validate a colorimetric enzymatic method of kinetic analysis developed with Cuban-made reagents, in order to determine the serum and urine creatinine for future application in the clinical laboratories.
Methods: the enzymatic method is used for kinetic determinations based on the spectrophotometric measurement at 550nm wavelength of the formed quinoneimine. The validation evaluated the parameters specificity, linearity, precision, accuracy, detection and quantitation limits in compliance with the present Cuban regulations.
Results: the method was specific for the suggested objective; provided linear response from 26,52 to 2 652 µmol/L in serum and from 884 to 26 520 µmol/L in urine; had great precision and accuracy and its detection and quantitation limits were adequate.
Conclusions: since the evaluated method fulfilled the present regulatory demands, it may be used in the domestic clinic laboratories to determine serum and urine creatinine.

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