medigraphic.com
ISSN 1561-3054 (Electronic)

2015, Number 3

<< Back Next >>

Rev Cubana Med Trop 2015; 67 (3)

In vitro evaluation of marine invertebrates extracts against Leishmania amazonensis

Piñón TA, García PM, Mendiola MJ, Pérez HA, Monzote FL

Language: Spanish
References: 18
Page:
PDF size: 161.81 Kb.


ABSTRACT

Leishmaniasis is a parasitosis caused by protozoa of the genus Leishmania. It is endemic in 98 countries, where the disease incidence is about two million new cases per year. The drugs used in the disease’s treatment have numerous toxic effects, high cost and a long application period; besides, the emergence of resistance to them is also added. Natural medicine and the use of natural resources are used as alternatives to current therapy. The study aims to evaluate the antileishmanial activity of 15 marine invertebrates extracts. For this, growth inhibition caused by the extracts against Leishmania amazonensis promastigotes and amastigotes was determined, together with its cytotoxicity against BALB/c mice peritoneal macrophages. Among the evaluated products, only four (26.7%) caused parasite growth inhibition, while only the Echinometra lucunter extract showed selectivity (SR=5) and activity against intracellular amastigotes (CI50 = 28.1 ± 6.0 mg/ml). These promising results provide insights about the possibility of using Echinometra lucunter extract as an antileishmanial agent.


REFERENCES

  1. Alvar J, Velez ID, Bern C. Leishmaniasis worldwide and global estimates of its incidence. PloS ONE. 2012;7:1-12.

  2. Castillo E, Dea-Ayuela MA, Bolás-Fernández F, Rangel M, González-Rosende ME. The kinetoplastid chemotherapy revisited: current drugs, recent advances and future perspectives. Curr Med Chem. 2010;17(33):4027–51.

  3. Cunha LC. Apropolisno combate a tripanosomatídeos de importancia médica: uma perspectiva terapéutica para doença de Chagas e leishmaniose. Pat Trop. 2011;40(3):105-24.

  4. Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicine. 2005;12(6-7):514-35.

  5. Faulker DJ. Marine natural products. Nat Prod Rep. 1998;15:113-58.

  6. Lam KS. Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol. 2006;9:245-51.

  7. Sima P, Vetvicka V. Bioactive substances with anti-neoplastic efficacy from marine invertebrates: Porifera and Coelenterata. World J Clin Oncol. 2011;2:355-61.

  8. Brito IM. Asteróides e equiniodes do Estado da Guanabara a adjacencias. Bolm Mus Nac Ser Zool. 1968;260:1-51.

  9. Blunt JW, Copp BR, Munro MHG, Northcote PT, Prinsep MR. Marine natural products. Nat Prod Rep. 2006;23:26-78.

  10. Kelecom A. Chemistry of Marine Natural Products: Yesterday, Today and Tomorrow. An Acad Bras Cienc. 1999;71:249-63.

  11. Capon RJ. Marine bioprospecting-trawling for treasure and pleasure. Eur J Org Chem. 2001;2001:633-45.

  12. Haefner B. Drugs from the deep: marine natural products as drug candidates. Drug Discov Today. 2003;8:536-44.

  13. García M, Monzote L. Marine Products with Anti-Protozoal Activity: A Review. Curr Clin Pharm. 2014;9:11.

  14. García M, Monzote L, Castañeda O, García N, Pérez A. Antileishmanial activity for six extracts from marine organisms. Rev Cub Med Trop. 2012;64(1):61-4.

  15. Seleghim MHR, Lira SP, Kossuga MH, Batista T, Berlinck S, Hadju E, et al. Antibiotic, cytotoxic and enzyme inhibitory activity of crude extracts form Brazilian marine invertebrates. Bras J Pharmacog. 2007;17(3):287-318.

  16. Tommasi L. Lista dos equinoides recentes do Brasil. Ser Ocean Biol. 1966;11(1):1- 50.

  17. Bernasconi I. Equinoideos y Asteroideos de la colección del Instituto Oceanográfico de la Universidad de San Pablo. Bolm Inst oceanogr S Paulo. 1955;6(1-2):51-77.

  18. Hendler G. Sea stars, sea urchins, and allies: echinoderms of Florida and the Caribbean. Smith Inst Press. 1995;1:197-249.




2020     |     www.medigraphic.com