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2015, Number 2

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Cuba y Salud 2015; 10 (2)

Microbiological diagnosis of the pleural fluids performed at “Juan Manuel Márquez”Pedriatic Hospital, 2009-2013

Vila AY, Valdés-Dapena VMM, Medell GM, Monzote LA, Díaz NL, Toraño PG

Language: Spanish
References: 30
Page: 10-16
PDF size: 1265.08 Kb.


ABSTRACT

Objective: To identify the agents causing the pleural effusion and determine the antimicrobial susceptibility of the isolations obtained from the culture of pleural fluids performed at “Juan Manuel Márquez” Pediatric Hospital from January 2009 to December 2013.
Method: A prospective observational study which included all the pleural liquids that were sent to the Microbiology laboratory during the periodstudied (N= 245).The process and identification of isolated microorganisms were carried out through conventional and fast diagnosis methods. Antimicrobial susceptibility was studied by the methods of microdilution in culture, E-test and diffusion in agarby disc(Bauer-Kirby).
Results: Positivity obtained was18,8%. Streptococcus pneumoniae (39,1%), predominated, all those isolationswere sensitive to penicillin¸ serotypes involved comprised were: 14, 9V, 6B, 3, 19, 19F. Staphylococcus aureus followed the former in frequency order, with predominance of strains that are resistant to methicillin (SARM; 19,6%). Emergency of thephenomenon of resistance inducible toclindamycin in both microorganisms was confirmed. The percentage of the othermicroorganisms identified was low.
Conclusions: S. pneumoniae and S. aureus were found as the principal agents causing the pleural effusioncoming from the community. The study of antimicrobial susceptibility offers valuable nformation for the management and empirical treatment of complicated pneumonias acquired in hospitalization.


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