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2015, Number 3

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Cuba y Salud 2015; 10 (3)

Using biotyping method to differentiate strains of Escherichia coli isolated from urine samples of pediatric patients

Hernández RE, Valdés-Dapena VMM, Torres MK, Vila AY
Full text How to cite this article

Language: Spanish
References: 9
Page: 21-24
PDF size: 355.83 Kb.


Key words:

Escherichia colibiotype, urinary tract infection.

ABSTRACT

Objetive: To introduce in the clinical diagnosis of urinary infections the biotyping strains of Escherichia coli identified in urine samples.
Materials and Methods: A retrospective observational study was conducted during the period from October 2012 to September 2013, at “Juan Manuel Márquez”. Pediatric Teaching Hospital The work included 539 strains of Escherichia coli isolated from urine samples obtained from patients suspected of suffering from a urinary tract infection. In these strains were applied the biotyping scheme described by Crichton and Old in 1993, which allowed the differentiation of this microorganism in different biotypes.
Results: The presence of 16 different biotypes of Escherichia coli strains studied was demonstrated. Biotypes 1 and 10 were the most identified with 20.59 and 17.62%, respectively.
Conclusions: According to the one described in the literature, the biotyping scheme used, shows its usefulness for discrimination in a wide range of biotypes of Escherichia coli recovered from urine samples obtained from patients with a clinical suspicion of infection urinary tract.


REFERENCES

  1. Koyle MA, Shifrin D. Issues in Febrile Urinary Tract Infection Management. Pediatr Clin N Am 2012; 59: 909–922.

  2. Newman DH; Shreves AE; Runde DP. Pediatric Urinary Tract Infection: Does the Evidence Support Aggressively Pursuing the Diagnosis?. Ann Emerg Med 2013;61:559-565.

  3. Bhat RG, Katy TA, Place FC. Pediatric Urinary Tract Infections. Emerg Med Clin N Am 2011; 29:637–653.

  4. Puñales Medel I, Monzote López A, Torres Amaro G, Hernández Robledo E. Etiología bacteriana de la infección urinaria en niños. Rev Cubana Med Gen Integr vol.28 no.4 Ciudad de La Habana oct.-dic. 2012

  5. Barber AE, Norton JP, Spivak AM, Mulvey MA. Urinary tract infections: current and emerging management strategies. Clin Infect Dis 2013;57(5):719–24.

  6. Schwartz DJ, Chen SL, Hultgren SJ, Seed PC. Population dynamics and niche distribution of uropathogenic Escherichia coli during acute and chronic urinary tract infection. Infect Immun 2011;79:4250-4259.

  7. Köhler CD, Dobrindt U. What defines extraintestinal pathogenic Escherichia coli? Int J Med Microbiol 2011;301:642-7.

  8. Crichton PB, Old DC. A miniaturized biotyping system for strain discrimination in E.coli. Epidemiol Infect 1993;111:81-88

  9. Hernández E. Caracterización fenotípica de cepas de Escherichia coli uropatógenas. [Trabajo para optar por el título de Máster en Bacteriología-Micología]. La Habana. Instituto de Medicina Tropical ¨Pedro Kourí¨;2002.




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Cuba y Salud. 2015;10