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2016, Number 4

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AMC 2016; 20 (4)

Biofilm detection in negative coagulasa staphylococcus and clinical epidemiological variables

Aties LL, del Río CG, Escalona VR, Brossard AG, Figueredo AIC, Duret GY
Full text How to cite this article

Language: Spanish
References: 15
Page: 351-358
PDF size: 732.51 Kb.


Key words:

biofilms, staphylococcus, agar/isolation & purification, epidemiologic factors, observational study.

ABSTRACT

Background: the capacity of some stumps of negative coagulase staphylococcus to produce biofilm has been considered by some authors as index of virulence and it is useful to differentiate them of those polluting stumps.
Objective: to determine the relationship between the biofilm production for stumps of negative coagulase staphylococcus and epidemic clinical variables.
Methods: an observational, analytical cross-sectional study was conducted in stumps of negative coagulase staphylococcus and the relationship between the biofilm production and the selected clinical-epidemic variables of the infected patients, in the Microbiology laboratory in "Saturnino Lora” hospital in Santiago de Cuba, from January to December 2012.
Results: the age groups who had more isolation to negative coagulase staphylococcus, in male and female sex was the 50-59 and the over 60 one. Regarding the non-communicable chronic diseases associated presented in the patients studied, it was observed that the highest number of affected people was those that presented diabetes mellitus and chronic nephropathies. The biggest figure of isolations to negative coagulase staphylococcus corresponded to the nephrology service. The antimicrobials that showed smaller resistance percentage were Vancomycin, Chloramphenicol, Novobiocin, and Doxicicycline.
Conclusions: the infection for negative coagulase staphylococcus is frequent in patient with advanced age, and diabetes mellitus and nephropathies are factors to keep in mind. Finally it was demonstrated that the red congo agar means, for their simplicity, high sensibility and specificity can be added to the diagnostic arsenal in our laboratories for the biofilm detection.


REFERENCES

  1. Grzebyk M, Brzychczy WM, Piotrowska A, Krzyściak P, Heczko PB, Bulanda M. Phenotyp-ic evaluation of hydrophobicity and the ability to produce biofilm in coagulase-negative staphylococci isolated from infected very-low-birthweight newborns. Med Dosw Mikrobiol. 2013;65(3):149-59.

  2. Blackledge MS, Worthington RJ, Melander C. Biologically inspired strategies for combating bacterial biofilms. Curr Opin Pharmacol. 2013 Oct;13(5):699-706.

  3. França A, Carvalhais V, Maira-Litrán T, Vilano-va M, Cerca N, Pier G. Alterations in the Staphylococcus epidermidis biofilm transcrip-tome following interaction with whole human blood. Pathog Dis. 2014 Apr;70(3):444-8.

  4. Salas-Sánchez OA, Rivera Morales I. Inciden-cia de infecciones relacionadas a catéteres ve-nosos centrales (CVC) en la Unidad de Cuida-dos Intensivos (UCI) de un hospital universita-rio. Rev médica universitaria. 2010;12(47):91-95.

  5. Rivera Castrillón LE, Ramos Palma A, Desga-rennes Padilla MC. Importancia de las biopelí-culas en la práctica médica. Rev Mex Derma-tología [Internet]. 2010 [citado 2 May 2013];54(1):[aprox. 10 p.]. Disponible en: http://www.medigraphic.com

  6. Silva Ferrera J, Rizo Rodríguez R, Castañeda Márquez V, Hing León JR. Prevalencia y causas de la insuficiencia renal crónica en 2 áreas de salud de Santiago de Cuba. MEDISAN [Internet]. 2013 [citado 2 May 2013]; 12(2):[aprox. 10 p.]. Disponible en: http://www.imbiomed.com.mx/1/1/articulos.php?met-hod=showDetail&id_articulo=54450&id_seccion=636&id_ejemplar=5518&id_revista=80

  7. Planet PJ, Larussa SJ, Dana A, Smith H, Xu A, Ryan C, et al. Emergence of the Epidemic Methicillin-Resistant Staphylococcus aureus Strain USA300 Coincides with Horizontal Transfer of the Arginine Catabolic Mobile Ele-ment and speG-mediated Adaptations for Sur-vival on Skin. MBio. 2013 Dec 17;4(6):e00889-13.

  8. Atshan SS, Shamsudin MN, Lung LT, Sekawi Z, Ghaznavi-Rad E, Pei CP. Comparative char-acterisation of genotypically different clones of MRSA in the production of biofilms. J Biomed Biotechnol. 2012;2012:417247.

  9. Haniagua-Contreras G, Monroy-Pérez E, Gutié-rrez-Lucas R, Sainz-Espuñes T, Bustos-Martínez J, Vaca S. Genotypic characterization of methicillin-resistant Staphylococcus aureus strains isolated from the anterior nares and catheter of ambulatory hemodialysis patients in Mexico. Folia Microbiol (Praha). 2014 Jul;59(4):295-302.

  10. Babra C, Tiwari J, Costantino P, Sunagar R, Isloor S, Hegde N, et al. Human methicillin-sensitive Staphylococcus aureus biofilms: potential associations with antibiotic re-sistance persistence and surface polysaccha-ride antigens. J Basic Microbiol. 2014 Jul;54(7):721-8.

  11. Guembe Ramírez M. Diagnóstico Micro-biológico de la Infección relacionada con el Catéter [tesis doctoral]. Madrid: Universidad Complutense; 2010.

  12. Yousefi M, Pourmand MR, Fallah F, Hashemi A, Mashhadi R, Nazari-Alam A. Characteriza-tion of Staphylococcus aureus Biofilm For-mation in Urinary Tract Infection. Iran J Pub-lic Health. 2016 Apr;45(4):485-93.

  13. Pascual A, Cercenado E, Salavert M, Elías García-Sánchez J, Eiros JM, Liñares J, et al. Update on pathogenesis and diagnosis of in-travascular catheter-related infections. Enferm Infecc Microbiol Clin. 2011 Mar;29 Suppl 4:16-21.

  14. Cha JO, Yoo JI, Yoo JS, Chung HS, Park SH, Kim HS, et al. Investigation of Biofilm For-mation and its Association with the Molecular and Clinical Characteristics of Methicillin-resistant Staphylococcus aureus. Osong Pub-lic Health Res Perspect. 2013 Oct;4(5):225-32.

  15. Boase S, Foreman A, Cleland E, Tan L, Mel-ton-Kreft R, Pant H, et al. The microbiome of chronic rhinosinusitis: culture, molecular di-agnostics and biofilm detection. BMC Infect Dis. 2013 May 8;13:210.




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