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2016, Number 5

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Rev ADM 2016; 73 (5)

Anti-inflammatory effect of caffeic acid in an experimental model of pulpitis in guinea pigs

Morones AJD, Macías HSI, Villanueva LGC, Aragón FM

Language: Spanish
References: 33
Page: 250-254
PDF size: 306.80 Kb.


ABSTRACT

Background: In experimental models, caffeic acid has proven to have anti-inflammatory properties through the modulation of the arachidonic acid metabolic cascade and of immune responses, effects which could be replicated in local inflammation sites, such as in the case of acute reversible pulpitis, the primary cause of which is dental caries. Objective: To characterize the anti-inflammatory properties of caffeic acid and compare these with those of Indomethacin in an experimental model of pulpitis in guinea pigs. Material and methods: An experimental study involving the teeth of 16 male guinea pigs was performed. These were randomly assigned to one of four groups: Group I: No pulpitis or treatment; Group II: Pulpitis untreated; Group III (experimental): Pulpitis treated with caffeic acid; and Group IV (comparative): Pulpitis treated with Indomethacin. The specimens were sacrificed at 24 hours after pulpitis induction. A qualitative histopathological analysis was performed by quantifying the inflammatory infiltrate. For the statistical analysis, the mean and standard deviation were obtained and the Wilcoxon, Mann-Whitney U, and Kruskal-Wallis tests used. Results: Caffeic acid completely inhibited macrophages and partially inhibited lymphocytes and polymorphonuclear cells (p ‹ 0.05), with no statistically significant difference with Indomethacin (p › 0.05). Conclusions: The effectiveness of caffeic acid in reducing the inflammatory process was comparable to that of indomethacin, by inhibiting lymphocytes, polymorphonuclear neutrophils and, above all, macrophages. Studies are needed in humans, given that caffeic acid offers a viable alternative for treating local inflammatory processes such as dental pulpitis.


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