Alfaro CJC, García EG, Wong GRA, Téllez IG

Language: English/Spanish
References: 21
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ABSTRACT

Broiler chicks were treated prophylactically with the soluble products from concanavalin A-stimulated T-lymphocytes from Eimeria tenella-infected chickens in order to study the effect of such prophylactic treatment on organ invasion by Salmonella enteritidis. Chicks were randomly assigned in three groups: Group A) experimental group (S. enteritidis-infected, treated): at one day of age, chicks were injected intraperitoneally with E. tenella-immune lymphokines, and thirty minutes after the lymphokine injection all chicks were challenged per os with 105 colony-forming units of S. enteritidis; Group B) the positive control group (S. enteritidis-infected, not treated) and Group C) the negative control group (S. enteritidis-not infected, not treated). At 24 hours post-challenge, all chicks were euthanized; liver, spleen and cecal tonsils were collected. Isolation of S. enteritidis was attempted from these tissues. The treatment of chicks with lymphokines resulted in reduction of S. enteritidis isolation from liver-spleen (P ‹ 0.05) but not from cecal tonsils (P›0.05). These results demonstrate that the prophylactic treatment with E. tenella-immune lymphokines reduces the S. enteritidis organ invasion but not the intestinal colonization in neonatal broiler chicks.

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