medigraphic.com
ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2016, Number 4

<< Back Next >>

Rev Mex Patol Clin Med Lab 2016; 63 (4)

Epidemiology of respiratory infections in pediatric patients tested by PCR multiplex

Ojeda PS, Munive LR, Moreno LLC, Torres GA, Melgar MV

Language: Spanish
References: 9
Page: 190-195
PDF size: 243.06 Kb.


ABSTRACT

Background: Mainly viral agents cause acute respiratory tract infections they represent in our country one of the main causes of mortality. The most reliable screening test is polymerase chain reaction (PCR); with the advantage that it can detect multiple agents with a single sample simultaneously. Objectives: Descrive the epidemiology of acute respiratory infections in pediatric patients of ABC Medical Center who where tested by multipanel respiratory FilmArrayTM. Material and methods: We performed a cross sectional, retrospective, observational, epidemiologic study in patients whose age were under 18 years old with acute respiratory tract infections and had nasopharyngeal mucus by the respiratory panel FilmArrayTM for the detection of an etiologic agent. Results: Median age of patients was 1.6 years. Most frequently detected viruses where: RSV in 34%, rhinovirus 27.8%, metapneumovirus 7.7% and parainfluenza virus 3 7.7%. The season in which more detections where observed was fall, predominating RSV (47.8%). During winter the most frequent viruses were influenza B in 5.6%, influenza A 3%, A H1 0.5% and A H3 1.5%. Conclusions: Respiratory multipanel FilmArrayTM could identify the most frequent pathogens in pediatric patients with respiratory symptoms.


REFERENCES

  1. Kim JK, Jeon JS, Kim JW, Rheem I. Epidemiology of respiratory viral infection using multiplex rt-PCR in Cheonan, Korea (2006-2010). J Microbiol Biotechnol. 2013; 23 (2): 267-273.

  2. Ellis C, Misir A, Hui C, Jabbour M, Barrowman N, Langill J et al. Detection of respiratory viruses and bacteria in children using a twenty-two target reverse-transcription real-time PCR (RT-qPCR) panel. World J Pediatr. 2016; 12 (2): 183-189.

  3. Ruggiero P, McMillen T, Tang YW, Babady NE. Evaluation of the BioFire FilmArray respiratory panel and the GenMark eSensor respiratory viral panel on lower respiratory tract specimens. J Clin Microbiol. 2014; 52 (1): 288-290.

  4. Xu M, Qin X, Astion ML, Rutledge JC, Simpson J, Jerome KR et al. Implementation of filmarray respiratory viral panel in a core laboratory improves testing turnaround time and patient care. Am J Clin Pathol. 2013; 139 (1): 118-123.

  5. Anderson TP, Werno AM, Barratt K, Mahagamasekera P, Murdoch DR, Jennings LC. Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens. J Virol Methods. 2013; 191 (2): 118-121.

  6. Vizcarra-Ugalde S, Rico-Hernández M, Monjarás-Ávila C, Bernal-Silva S, Garrocho-Rangel ME, Ochoa-Pérez UR et al. Intensive care unit admission and death rates of infants admitted with respiratory syncytial virus lower respiratory tract infection in Mexico. Pediatr Infect Dis J. 2016; 35 (11): 1199-1203.

  7. Tulloh RM, Bury S. Prevention and prophylaxis of respiratory syncytial virus in pediatric cardiology: a UK perspective. Future Cardiol. 2014; 10 (2): 235-242.

  8. Prado MA, Le Corre N, Viviani T, Perret C. Endocarditis caused by Streptococcus pneumoniae in children: case report and review. Rev Chilena Infectol. 2005; 22 (4): 361-367.

  9. Brink AJ, Van Wyk J, Moodley VM, Corcoran C, Ekermans P, Nutt L et al. The role of appropriate diagnostic testing in acute respiratory tract infections: An antibiotic stewardship strategy to minimise diagnostic uncertainty in primary care. S Afr Med J. 2016; 106 (6): 30-37.




2020     |     www.medigraphic.com