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Revista Mexicana de Patología Clínica y Medicina de Laboratorio

ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio
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2004, Number 4

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Rev Mex Patol Clin Med Lab 2004; 51 (4)

Determination of antibiotypes of Pseudomonas aeruginosa in environmental study in the Hemodialysis Unit of the Hospital “Dr. Miguel Enríquez”

Delgado PML, Rodríguez PAU, Moreno CEE, Debrosse BZ
Full text How to cite this article

Language: Spanish
References: 14
Page: 226-230
PDF size: 47.04 Kb.


Key words:

Hemodialysis, Pseudomonas aeruginosa, microbiological surveillance.

ABSTRACT

In the Hemodialysis Unit of the Hospital “Dr. Miguel Enríquez”, was carried out an environmental study to detecting the sources contaminated by Pseudomonas aeruginosa. They took 59 samples, 10 of them corresponding disinfectant solutions, 8 of water of treatment plant, 2 of material sterile, 16 of hands of staff nurse and 23 in different elements of the artificial kidneys. The isolated of Pseudomonas aeruginosa were identified and confirmed in the Provincial Center of Hygiene and Epidemiology and to the isolated they were carried Out the determination of the antimicrobial susceptibility by the diffusion method in agar, with the objective of determining the present antibiotypes as epidemic marker of this species. One could obtain isolations of Pseudomonas aeruginosa in 14 samples. 85,7% of them belongs together with the different elements of the artificial kidneys and 35,7 remaining of the isolations was obtained starting from samples of water of the plant of treatment. 100% of the stumps proven to determine their resistance pattern in vitro, they went resistant to the antibiotics: kanamicine, sulfametoxazol, chloramphenicol, tetracicline, streptomycin and carbeniciline. The approaches referred to the multirresistance found in the stumps of Pseudomonas aeruginosa of origin nosocomial, they have become evident in this study. It is important to stand out that independently of the origin of the one that these stumps were isolated, its behavior as for the antibiotic resistance was the same one, and it was a single antibiotype of this species. Aspect that charges relevance considering that Pseudomonas aeruginosa can end up contaminating all the branches of these equipments, ending up being responsible for serious buds of nosocomial infection in this patient. You could also evidence the multirresistance in 100% of the isolated stumps of Pseudomonas aeruginosa and to detect the presence of a single antibiotype of this species in the Unit of Hemodialysis of the Hospital “Dr. Miguel Enríquez”.


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Rev Mex Patol Clin Med Lab. 2004;51