2016, Number 4
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ABSTRACTIntroduction. Acinetobacter baumannii infections frequently occur as outbreaks with high mortality rate due to multidrug resistance. Molecular analysis of outbreaks has shown high genetic diversity and the presence of different epidemic clones, but these have not been identified in hospitals of Mexico City. Aim. Determine the genetic variability of A. baumannii in a hospital in Mexico City. Material and methods. The DNA of 12 multiresistant isolates of A. baumannii was amplified by RAPD using four random oligonucleotides. From the polymorphic patterns a dendrogram was constructed by UPGMA, a multidimensional diagram of major components and cophenetic correlation coefficient (CCCr) was calculated. Genetic diversity was estimated by Shannon index (I) and Nei genetic diversity, using allele frequencies, expected heterozygosity for each population and the average heterozygosity. The reproduction structure was determined by the rate of association (IA). Results. The dendrogram showed the formation of six groups (I-VI), supported by bootstrap high values (› 73%), this group was corroborated by the major components diagram. Estimates of genetic diversity showed that groups IIb and III were the most and least variables, respectively. The IA values indicated a clonal reproduction system for IIa and IIb subgroups, and recombinant for III and VI groups. Conclusions. Genetic variability among isolates of A. baumannii revealed the presence of nine epidemic clones in the hospital.
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