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2016, Number 3

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Rev Cubana Plant Med 2016; 21 (3)

Phytochemical study and anti-inflammatory activity of leaves, flowers and fruits of Bejaria resinosa Mutis ex L. (pegamosco)

Matulevich PJA, Gil AE, Ospina GLF

Language: Spanish
References: 30
Page: 332-345
PDF size: 180.16 Kb.


ABSTRACT

Introduction: Bejaria resinosa Mutis ex L. is a plant species known as pegamosco in Colombia. It is used by several communities to catch insects and to treat respiratory disorders. Few chemical and biological studies have been conducted about this species.
Objectives: Contribute to the phytochemical study of leaves, flowers and fruits of B. resinosa (Ericaceae) and evaluate its anti-inflammatory activity.
Methods: Leaves, flowers and fruits were extracted separately by cold maceration with petroleum ether and 96% ethanol. The extracts obtained were fractioned using liquid/liquid partition and chromatographic methods. Their anti-inflammatory activity was evaluated with the mouse model of ear edema induced by 12-tetradecanoyl phorbol 13-acetate (TPA). Structural characterization of the compounds isolated was based on GC-MS and NMR techniques (experiments 1H, 13C, COSY, J-MOD, HSQC and HMBC).
Results: Separation of extracts and fractions by thin-layer and preparative column chromatography made it possible to obtain a mixture of triterpene compounds made up of germanicol, α-amyrin and β-amyrin, and isolate lupeol, methyl salicylate, 3,5,7,3´,4´-pentahydroxyflavone (quercetin), 3,5-dihydroxy-6,7,8-trimetoxyflavona and 3,5,7,3´,4´-pentahydroxyflavanol. It was found that the fraction containing the mixture of triterpenes and quercetin had an anti-inflammatory effect above 65%.
Conclusions: The phytochemical study of the plant species B. resinosa revealed similarities between the chemical composition of the different organs, since common secondary metabolites were found in leaves, flowers and fruits, such as the mixture of triterpenes, lupeol and quercetin. It was also established that the mixture of triterpenes and quercetin is a strong anti-inflammatory agent, for it significantly reduced the mouse ear edema caused by TPA with inhibition percentages close to those of the drug of reference indometacin.


REFERENCES

  1. Luteyn J, Pedraza P. The New York Boanical Garden. [Internet]; 2007. Disponible en: www.nybg.org/bsci/res/lut2.

  2. Clemants S. Two New Species of Bejaria (Ericaceae) from South America. Brittonia. 1991;43(3):171-77.

  3. Bussmann RW, Malca-Garcia G, Glenn A, Sharon D, Chait G, Diaz D, et al. Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterial remedies. Journal of Ethnopharmacology. 2010;132:101-08.

  4. Luteyn JL. Ericaceae New York: Organization for Flora Neotropica: New York Botanical Garden; 1995.

  5. Bilbao M. Análisis Fitoquimico Preliminar Armenia: Oficina de publicaciones Universidad del Quindio; 1997.

  6. Sanabria A. Análisis fitoquímico preliminar Bogotá: Universidad Nacional de Colombia; 1983.

  7. De Young L, Kheifets J, Ballaron S, Young J. Edema and cell infiltration in the forbol esther treated mouse ear are temporally separated and can be differentially modulated by pharmacologic agents. Agents and actions. 1989;26:335-41.

  8. Payá M, Ferrándiz ML, Sanz MJ, Bustos G, Blasco R, Rios JL, et al. Study of the antioedema activity of some seaweed and sponge extracts from the mediterranean coast in mice. Phytotherapy Research. 1993:7:159-62.

  9. Garcia MD, Fernandez M, Alvarez A, Saenz M. Anticonceptive and antiinflamatory effect of the aqueous extract from leaves of pimenta racemosa var ozua (Mirtaceae). Journal of Ethnopharmacology. 2004; 91:69-73.

  10. Argay G, Kalman A, Kapor A, Ribar B, Petrovic S, Gorunovid M. Crystal structure of a mixture of lupeol acetate tautomers isolated from Hieracium plumulosum A. Kerner, Asteraceae. Journal of Molecular Structure. 1997;435:169-79.

  11. Bracho J, Rodriguez C, Llanez F. Triterpenos pentacíclicos en propóleo. Revista de la Sociedad Química del Perú. 2009;75:439-52.

  12. Costa M, Chaves M. Constituientes Quimicos e atividade antioxidante de extratos das folhas de Terminalia fagifolia Mart. et Zucc. Quimica Nova. 2009;32:1509-12.

  13. Harborne J, Williams C. A chemotaxonomic survey of flavonoids and simple phenols in leaves of the Ericaceae. Botanical J. Linn. 1973;66:37-54.

  14. Ueda K, Herz W, Pachter I. The Triterpenes of Befaria racemosa. Deparment of chemistry,The Florida State University. 1961;271-72.

  15. Font Quer P. Plantas Medicinales, El Dioscórides Renovado Barcelona: Ediciones Península; 2001.

  16. Talapatra B, Patra A, Sunil T. Terpenoids and alkaloids of the leaves of Tabernaemontana coronaria. Phytochemistry. 1975;14(7):1652-53.

  17. Bhattacharyya J, Barros C. Triterpenoids of Cnidosculus urens. Phytochemistry. 1985;25(1):274-76.

  18. Urrego O. Contribución al estudio fitoquimico de las especies Bejaria congesta, Bejaria aestuans y Bejaria resinosa. Bogota: 1988.

  19. Prakash C, Bing-Nan Z, Shigie G, Shannon J, Sidney M. New lupane triterpenoids from Solidago canadensis that inhibit the lyase activity of DNA polymerase β. Bioorganic & Medicinal Chemistry. 2004;12(23):6271-75.

  20. Godkil G, Topcu G, Sonmez U, Ulubelen A. Terpenoids and flavonoids from Salvia cyanescens. Phytochemistry. 1997;46(4):799-800.

  21. Nguyen A, Malonne H, Duez P, Vanhaelen-Fastrea R, Vanhaelen M, Fontaine J. Cytotoxic constituents from Plumbago zeylanica. Fitoterapia. 2004;75(5):500-04.

  22. Robinson F, Martel H. Betulinic acid from Arbutus menziesii. Phytochemistry. 1970;9(4):907-09.

  23. Zhang B, LI HE X, Ding Y, Du G. Gaultherin, a natural salicylate derivative from Gaultheria yunnanensis: Towards a better non-steroidal anti-inflammatory drug. European Journal of Pharmacology. 2006;530:166-171.

  24. Gournelis DC. Flavonoids of Erica verticillata. Journal of Natural Products. 1995;58:1065-69.

  25. Tsanova F, Ribarova F, y Gerova M. (+)-Catechin and (-)-epicatechin in Bulgarian fruits. Journal of Food Composition and Analysis. 2005;18:691-698.

  26. Vasco C, Riihinen K, Ruales J, Kamal A. Chemical composition and phenolic compound profile of Mortiño (Vaccinium floribundum Kuth). Journal of Agricultural and Food Chemistry. 2009;57:8274-8281.

  27. Jimenez M, Chilpa R, Apan T, Lledias F, Hansberg W, Arrieta D. Antiinflammatory activity of cacalol and cacalona sesquiterpenes isolated from Psacalium decompositum. Journal of Ethnopharmacology. 2006;105:34-38.

  28. Gonzalez E, Villca T, Loza R. Evaluacion de la actividad antiinflamatoria de ocho especies del genero Baccharis: B. articulata, B. dracunculifolia, B. salicifolia, B. ulcina, B. latifolia, B. pentlandii, B. obtusifolia, B. subalata. Revista Boliviana de Química. 2007;24:41-44.

  29. Selloum L, Bouriche H, Tigrine C, Boudoukha C. Antiinflammatory effect of rutin on rat paw oedema, and on neutrophils chemotaxis and degranulation. URBAN & FISCHER. Exp Toxic Pathol. 2003;54:313-18.

  30. Coskun O, Kanter M, Armutcu F, Cetin K, Kaybolmaz B, Yazgan O. Protective effects of quercetin, a flavonoid antioxidant, in absolute etanol induced. Eur. J. Med. 2004;1:37-42.




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