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2017, Number 1

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Rev Cubana Invest Bioméd 2017; 36 (1)

Bioinspired chitosan-BSA fibers for applications in tissue engineering of the fibrous ring of intervertebral discs

Peniche AH, David L, Peniche CC, Osorio-Madrazo A

Language: Spanish
References: 19
Page: 1-11
PDF size: 241.20 Kb.


ABSTRACT

Introduction: Chitosan fibers are used to construct or reinforce scaffolds and supports for tissue engineering, improving their mechanical properties. Spinning in chitosan solution in interaction with proteins had not been performed before, and may be used to obtain bioinspired fibers of the chitosan-albumin complex with suitable properties and a morphology similar to the fibrous tissue found in intervertebral discs, useful for their potential repair.
Objectives: Applying a wet spinning technique, prepare and characterize bioinspired fibers of the chitosan complex with bovine serum albumin for potential use in tissue engineering of the fibrous ring of intervertebral discs.
Methods: A study was conducted of the experimental conditions required to obtain chitosan-protein complexes with dissolution properties suitable for spinning. Wet spinning was performed to obtain chitosan fibers with bovine serum albumin. These were characterized and their mechanical properties determined.
Results: The fibers obtained had a microfibrillar structure similar to natural chitin. The Fourier transform infrared spectra obtained by attenuated total reflection make evident the incorporation of bovine serum albumin into the chitosan fibers. Micromechanical assays revealed that the chitosan-albumin fibers display high Young's modulus values and resistance to fracture.
Conclusions: Chitosan-albumin fibers may be obtained by spinning in solution of chitosan-albumin complexes without the use of any crosslinking agent or posttreatment. The fibers have mechanical properties suitable for their use as reinforcement of hydrogels for application as biomaterials in tissue engineering of intervertebral discs. Bovine serum albumin was used as a globular protein model. The next step in the study will be the use of the collagen for the required application.


REFERENCES

  1. Iatridis JC, Nicoll SB, Michalek AJ, Walter BA, Gupta MS. Role of biomechanics on intervertebral disc degeneration and regenerative therapies: What needs repairing in the disc andwhat are promising biomaterials for its repair? Spine J. 2013;13(3):243-62.

  2. Bailey A, Araghi A, Blumenthal S, Huffmon GV. Prospective Multicenter, Randomized, Controlled Study of Anular Repair in Lumbar Discectomy. Spine. 2013;38(14):1161-9.

  3. O'Connell GD, Malhotra NR, Vresilovic EJ, Elliott DM. The Effect of Discectomy and the Dependence on Degeneration of Human Intervertebral Disc Strain in Axial Compression. Spine. 2011;36(21):1765-71.

  4. Pei BQ, Li H, Zhu G, Li DY, Fan YB, Wu SQ. The Application of Fiber-Reinforced Materials in Disc Repair BioMed Research International. 2013;2013:10. ID 714103.

  5. Smith LJ, Nerurkar NL, Choi KS, Harfe BD, Elliott DM. Degeneration and regeneration of the intervertebral disc: lessons from development. Dis Model Mech. 2011;4(1):31-41.

  6. O'Halloran DM, Pandit AS. Tissue-engineering approach to regenerating the intervertebral disc. Tissue Eng. 2007;13(8):1927-54.

  7. Schollmeier G, Lahr-Eigen R, Lewandrowski KU. Observations on fiber-forming collagens in the anulus fibrosus. Spine J. 2000;25:2736-41.

  8. Osorio-Madrazo A, Fratzl P, Davi L, Urban G, Montembault A, Crepet A, et al. Hydrogel nanocomposite biomaterials for intervertebral disc tissue engineering. Preparation, characterization and application. Bionanomaterials. 2015;16:236-55.

  9. Visser J, Melchels FPW, Jeo E, vanBussel EM, Kimpton LS, Byrne HM, et al. Reinforcement of hydrogels using three-dimensionally printed microfibers. Nature Communications 2015;6:1-10.

  10. Chanzy H. Chitin crystals. In: Domard A, Varum KM, Muzzarelli RAA, editors. Advances in Chitin Science Proceedings of the 7th International Conference on Chitin and Chitosan. Lyon: Jacques André Publisher; 1998. p. 11.

  11. Muzzarelli RAA. Chitin New York: Pergamon Press; 1977.

  12. Amidi M, Mastrobattista E, Jiskoot W, Hennink WE. Chitosan-based delivery systems for protein therapeutics and antigens. Adv Drug Deliv Rev. 2010;62:59-82.

  13. Yuan Y, Wan ZL, Yang XQ, Yin SW. Associative interactions between chitosan and soy protein fractions: Effects of pH, mixing ratio, heat treatment and ionic strength. Food Research International. 2014;55:207-14.

  14. Dresvyanina EN, Dobrovolskaya IP, Popryadukhin PV, Yudin VE, Ivankova EM, Elokhovskii VY, et al. Influence of spinning conditions on properties of chitosan fibers. Fibre Chemistry. 2013;44(5):280-3

  15. Notin L, Viton C, David L, Alcouffe P, Rochas C, Domard A, et al. Morphology and mechanical properties of chitosan fibers obtained by gel-spinning: Influence of the dry-jet-stretching step and ageing. Acta Biomaterialia. 2006;2:387-402.

  16. Bomshtein KG, Danilov VI, Pravetskii VN. Statics and dynamics of intervertebral discs. Mechanics of Composite Materials. 1980;15(4):419-23.

  17. Sato K, Kikuchi S, Yonezawa T. In vivo intradiscal pressure measurement in healthy individuals and in patients with ongoing back problems. Spine J. 1999;24:2468-74.

  18. Beachley V, Wen X. Fabrication of nanofiber reinforced protein structures for tissue engineering. Materials Science and Engineering C. 2009;29(8):2448-53.

  19. Dutov P, Antipova O, Varma S, Orgel JPRO, JD. Schieber Djp. Measurement of Elastic Modulus of Collagen Type I Single Fiber. PLOS ONE. 2016. DOI:101371/journalpone0145711.




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