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2018, Number 1

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AbanicoVet 2018; 8 (1)

Comparative evaluation of two methods of spermatic recovery of epididymis bovine post-mortem

Benítez-González E, Chamba-Ochoa H, Sánchez-Sánchez E, Luzón-Cevallos F, Sánchez-Carrillo J

Language: Spanish
References: 20
Page: 59-74
PDF size: 430.71 Kb.


ABSTRACT

Determine the appropriate method to recover bovine’s epididymal sperm in different post-mortem recovery times was the objective of the current studies. Testicles from 50 bulls of different breeds and ages, sanitary status, were obtained after the sacrifice of it in the slaughterhouse located in Loja city, Ecuador; testing two methods of seminal collection: retrograde wash and slicing. The testicles/epididymal were transported in 0.9% sodium chloride solution and storaged during 2, 4, 8, 12 and 24 hours at 5ºC, to test spermatic quality in relation with passed time from its sacrifice, to the sperm collection in the laboratory. The evaluated parameters were: mass motility, individual motility, percentage of viable sperm, and normal. In the analysis of the results descriptive statistics were used and for the variance analysis Tukey test were used to determine the statistical differences between averages. The epididymal spermatozoa showed: mass motility 60.4% ± 4.75; Individual motility 50.7% ± 4.75; Vitality 60.6% ± 3.85; Abnormalities 8.78% ± 1.4; there is no significant difference (p›0.05) between protocols, getting average volumes of 2.2±05 ml, concentration of 63.08% ± 2.05, the percentage of living sperm was more when the method of retrograde wash was used. It is concluded that is possible collect living sperm from the post-mortem epididymis bull’s tail, being its vitality directly proportional to the storage time.


REFERENCES

  1. Albers Alvarez MI, Barrios Arismendi DR. 2006. Movilidad individual de los espermatozoides epididimarios de toros post mortem obtenido mediante lavado retrógrado. Zootecnia Tropical. 24 (3), 267-279. http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0798-72692006000300006

  2. Anel L, Gerra C, Alvarez M, Anel E, Martinez A, Boixo C, Kaabi M, Herraez P, Paz P. 2002. Effect of postmortem interval in quality of epididymal spermatozoa in Iberian red deer (Cervus elaphus hispanicus). Theriogenology. 57: 577 (Abstr).

  3. Armas R, Fernández A, Vásquez C. Santiani AA. 2001. Determinación del tiempo máximo para recuperar y criopreservar espermatozoides obtenidos de la cola del epidídimo en caninos post orquiectomía. Revista de Investigaciones Veterinarias del Perú. 3 (22):199- 207. DOI: http://dx.doi.org/10.15381/rivep.v22i3.257 http://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/257

  4. Baracaldo M, Barth A, Bertrand W. 2007. Pasos para el congelamiento de semen bovino: desde la colección del semen hasta el almacenamiento en el tanque de nitrógeno líquido. www.ivis.org/newsletter/archives/apr07/apr3007es.htm

  5. Barbosa Luciano Munita, Kanazawa Mábilis Yumi, Peres Anelise Ribeiro, Ferreira de Souza Fabiana. 2012. Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem. Revista Brasileira de Ciencias Veterinarias. 3 (19):190-194. DOI: http://dx.doi.org/10.22409/rbcv.v19i3.69 http://www.rbcv.uff.br/rbcv/article/view/69

  6. Barrios AD. 2002. Evaluación de la calidad y capacidad fecundante de espermatozoides de la cola del epidídimo de toros Post Mortem. (U. C. Venezuela, Ed.) IX Congreso venezolano de Producción e Industria Animal. Valera, Venezuela.

  7. Barth A, Bo G, Tríbulo H. 2000. Curso de evaluación de toros y control de la calidad seminal. (U. c. Córdoba, Ed.).

  8. Castro JB, Casas VF, Souza FF. 2009. Viabilidade dos espermatozóides colhidos do epidídimo de touros 24 horas post-mortem. Resúmenes del Congresso Brasileiro de Reprodução Animal, Belo Horizonte, Brasil. 379 p.

  9. Celeghini E, Arruda R, Andrade A, Nascimento J, Raphaela C, Rodrigues P. 2008. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reproduction Science. 104:119-131. https://doi.org/10.1016/j.anireprosci.2007.02.001

  10. Chávez M. 2008. Comparación en pruebas microscópicas pre-congelación y post descongelación de semen de toros de lidia post mortem. Recuperado el 16 de febrero de 2015, de http://androvet.blogspot.com/

  11. Gonçalves PBD, Figueiredo JR, Freitas VJ. 2008. Biotécnicas aplicadas à reprodução animal (Segunda ed.). São Paulo, Brasil: Editora Roca. 408-410 p.

  12. Goovaerts IGF, Hoflack GG. Van Soom A, Dewulf J, Nichi M, Kruif de A, Bols PEJ. 2006. Evaluation of epididymal semen quality using the Hamilton-Thorne analyser indicates variation between the two caudae epididymides of the same bull. Theriogenology. 66 (2):323–330. DOI: http://dx.doi.org/10.1016/j.theriogenology.2005.11.018 http://www.theriojournal.com/article/S0093-691X(05)00522-4/abstract

  13. James AN, Green H, Hoffman S, Landry AM, Paccamonti D, Godke RA. 2002. Preservation of equine sperm stores in the epididymis at 4°C for 24, 28, 72 and 96 hours. Theriogenology. 58:401-404. https://doi.org/10.1016/S0093-691X(02)00883-X

  14. Martins CF, Rumpf R, Pereira DC, Dode MN. 2007. Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Animal Reproduction Science. 101:326-331. https://doi.org/10.1016/j.anireprosci.2007.01.018

  15. Martins CF, Driessen K, Melo Costa P, Carvalho-Neto JO, De Sousa R, Rumpf R, Dodec MN. 2009. Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 °C by different periods of time. Animal Reproduction Sciense. 116:50-57. https://doi.org/10.1016/j.anireprosci.2008.12.018 https://www.researchgate.net/profile/Jose_Carvalho33/publication/23963381_Recovery_cryopreservation _and_fertilization_potential_of_bovine_spermatozoa_obtained_from_epididymides_stored_at_5C_by_diff erent_periods_of_time/links/54f87b450cf210398e96a951/Recovery-cryopreservation-and-fertilizationpotential- of-bovine-spermatozoa-obtained-from-epididymides-stored-at-5C-by-different-periods-of-time.pdf

  16. Melo, C. M., Papa, F. O., Fioratti, E. G., Villaverde, A. I., Avanzi, B. R., & Monteiro, G. 2008. Comparison of three different extenders for freezing epididymal stallion sperm. Animal Reproduction Science. 3(107):331. https://doi.org/10.1016/j.anireprosci.2008.05.108

  17. Morillo M, Salazar S, Castillo E. 2012. Evaluación del potencial reproductivo del macho bovino. Instituto Nacional de Investigaciones Agrícolas. 60 p.

  18. Oyeyemi MO, Ubiogoro O. 2005. Espermiograma y Características Morfológicas de los Espermatozoides en el Testículo y Epidídimo del Verraco Grande Blanco en Nigeria. International Journal of Morphology. 23(3):237-238. http://dx.doi.org/10.4067/S0717- 95022005000300008 http://www.scielo.cl/scielo.php?pid=S0717- 95022005000300008&script=sci_arttext

  19. Reyes-Moreno, C., Boilard, M., & Sullivan, R., Sirard M. 2002. Characterization and identification of epididymal factors that protect ejaculated bovine sperm during in vitro storage. Biol. Reprod. 66(1):159-166. https://doi.org/10.1095/biolreprod66.1.159

  20. Rodríguez Márquez J, Madrid Bury N, Aixa U, Atilio Aranguren J, Quintero A. 2000. Análisis morfométrico del epidídimo en toros jóvenes mestizos 5/8 Holstein y 5/8 Pardo Suizo con testículos pequeños. (U. d. cientifica, Ed.) Revista científica, FCV-LUZ. 10(6):458-467. http://www.fcv.luz.edu.ve/images/stories/revista_cientifica/2000/06/articulo4.pdf




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