Specific antibody production against different life cycle stages during an experimental A. costaricensis infection in mice.

Elizabeth Abrahams-Sandí; Stefan Michael Geiger; Katherine Fernández-Quesada; Hartwig Schulz-Key

2005, Number 4

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Rev Biomed 2005; 16 (4)

**Specific antibody production against different life cycle stages during an experimental A. costaricensis infection in mice.**

Abrahams-Sandí E, Michael Geiger S, Fernández-Quesada K, Schulz-Key H

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Language: English
References: 54
Page: 239-246
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ABSTRACT

Objectives.- In the present study we aimed at investigating the humoral immune response against different Angiostrongylus costaricensis life-cycle stages- L1, L3, eggs and adult worms- during an experimental infection in C57BL/6 mice.
Materials and Methods.- C57BL/6 mice were experimentally infected with six third- stage larvae (L3) Angiostrongylus costaricensis. Blood samples from infected mice were obtained weekly by puncture of the retroorbital venous plexus and individual sera were stored at –20°C. PBS-soluble antigen extracts from adult worm, its excretory-secretory products, L1, L3, and eggs were prepared. Fractionation of all soluble antigen preparations by SDS-PAGE was performed and the antibody production in infected mice against all antigens was monitored during 9 weeks by ELISA.
Results and Discussion.- The production of different antibody isotypes was directed against all the different antigen preparations, with a predominance of IgG1 antibodies and a significant increase of IgA antibody levels after maturation of worms and egg deposition. The highest parasite-specific IgA antibody levels were detected against E/S- and L3-antigen at 2 and 3 weeks p.i., respectively. On the other hand, the highest IgG1 antibody response was measured against E/S- and egg-antigen at 3 weeks p.i. and remained high until the end of the experiment. Values for parasite-specific IgG_2a, determined by ELISA, reached only low levels against all antigen preparations. We propose further studies with human sera in order to investigate the usefulness of egg and E/S-antigen for the diagnosis of human abdominal angiostrongyliasis.

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