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TIP Revista Especializada en Ciencias Químico-Biológicas

2018, Number 1

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TIP Rev Esp Cienc Quim Biol 2018; 21 (1)

Chromium (VI) induces the mutation frequency and loss of heterocigocity in Saccharomyces cerevisiae

Santoyo G

Language: Spanish
References: 26
Page: 34-39
PDF size: 432.27 Kb.


ABSTRACT

Chromium (Cr) is one of the main pollutants in industrial waste, so it has been associated with various damages to human health. Therefore, in this work we analyze the effect of the hexavalent Cr on the frequency of spontaneous mutation (by using the forward mutation reporter CAN1) and the loss of heterozygosity in the yeast Saccharomyces cerevisiae, and in a mutant in the rev3 gene, which codes for the zeta polymerase. The results suggest that Cr (VI) can induce mutation frequency between 20 and 28-fold, at concentrations of 25 and 50 mM, respectively. This mutation frequency also increased in a rev3 mutant, although the same levels were not observed as in the wild strain, suggesting that in addition to rev3, there are other factors also participate during mutation induction in CAN1 marker. Cr also had an effect by increasing the heterozygosity loss of between 50 and 57-fold in the wild-type strain and the rev3 mutant, respectively. These results show that REV3 participates in the mutagenic effect caused Cr (VI), but not in the loss of heterozygosity, in yeast cells.


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